HMGCS1 antibody (AA 251-520)

Catalog No. ABIN7872930

The Rabbit Polyclonal anti-HMGCS1 antibody is suitable to detect HMGCS1 in samples from Human. It has been validated for WB, ELISA, IF, FACS and IHC (p).

Quick Overview for HMGCS1 antibody (AA 251-520) (ABIN7872930)

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HMGCS1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HMGCS1 Antibody

Binding Specificity: AA 251-520

Purpose: HMGCS1 Antibody

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids K251-H520) was used as the immunogen for the HMGCS1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HMGCS1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HMGCS1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HMGCS1

Target: HMGCS1 (3-Hydroxy-3-Methylglutaryl-CoA Synthase 1 (Soluble) (HMGCS1))

Alternative Name: HMGCS1

Background: Hydroxymethylglutaryl-CoA synthase or HMG-CoA synthase EC 2.3.3.10 is an enzyme which catalyzes the reaction in which acetyl-CoA condenses with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Cytoplasmic 3-hydroxy-3-methylglutaryl-CoA synthase (HMGCS1) catalyzes the first committed step of mevalonate pathway essential for cholesterol biosynthesis. HMGCS1 transcription is regulated by sterol levels. Studies showed that miR-223 reduces cholesterol biosynthesis by inhibiting HMGCS1 and methylsterol monooxygenase 1. In addition, activation of the EGFR family member ERBB4 induces the expression of SREBP-regulated genes (including HMGCS1) involved in cholesterol biosynthesis, suggesting a role of this enzyme in the metabolic re-programming in ERBB4-driven cancers.

UniProt: Q01581

Pathways: Regulation of Lipid Metabolism by PPARalpha