HTR3E antibody (AA 26-229)

Catalog No. ABIN7872988

The Rabbit Polyclonal anti-HTR3E antibody (ABIN7872988) specifically detects HTR3E in WB, ELISA and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for HTR3E antibody (AA 26-229) (ABIN7872988)

Target: HTR3E (Serotonin Receptor 3E (HTR3E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR3E antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HTR3E Antibody

Binding Specificity: AA 26-229

Purpose: HTR3E Antibody / 5-hydroxytryptamine receptor 3E

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HTR3E recombinant protein (Position: V26-R229) was used as the immunogen for the HTR3E antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HTR3E antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HTR3E antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HTR3E

Target: HTR3E (Serotonin Receptor 3E (HTR3E))

Alternative Name: HTR3E

Background: The HTR3E antibody targets 5-hydroxytryptamine receptor 3E, a serotonin receptor subunit encoded by the HTR3E gene. This protein belongs to the ligand-gated ion channel family and contributes to the formation of functional 5-HT3 receptor complexes that mediate fast excitatory neurotransmission in the central and peripheral nervous systems. 5-hydroxytryptamine receptor 3E does not form functional homopentamers by itself but modulates receptor properties when co-assembled with other 5-HT3 subunits such as HTR3A. The HTR3E antibody allows researchers to study this modulatory subunit's expression, localization, and physiological roles in serotonergic signaling.

5-hydroxytryptamine receptor 3E influences the biophysical and pharmacological characteristics of heteromeric 5-HT3 receptors. Its inclusion in the receptor complex alters ion conductance, ligand sensitivity, and desensitization kinetics. The HTR3E antibody supports detection of this subunit in intestinal epithelium, brainstem, and enteric neurons, where serotonergic signaling regulates motility, secretion, and sensory transmission. Expression in the gastrointestinal tract suggests a potential role in visceral sensory modulation and nausea control.

Polymorphisms in the HTR3E gene have been linked to irritable bowel syndrome, functional dyspepsia, and other serotonin-related gastrointestinal disorders. The HTR3E antibody facilitates analysis of protein expression in these contexts, helping to clarify the molecular basis of serotonin receptor diversity and disease susceptibility. In the nervous system, 5-hydroxytryptamine receptor 3E expression may fine-tune synaptic activity and contribute to central processing of nausea, anxiety, and emesis. Detection using the HTR3E antibody supports studies on how receptor composition affects neurotransmission and pharmacologic responses to 5-HT3 antagonists used in antiemetic therapy.

Beyond physiology, aberrant expression of 5-hydroxytryptamine receptor 3E has been observed in some cancers, suggesting roles in tumor cell signaling and proliferation. The HTR3E antibody allows evaluation of receptor expression in tumor tissues and cultured cells, supporting research into serotonin-mediated growth regulation. Functional studies continue to explore how HTR3E variants influence receptor pharmacodynamics and sensitivity to drugs such as ondansetron and granisetron.

The HTR3E antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry. It provides clear membrane and cytoplasmic staining consistent with receptor localization. NSJ Bioreagents provides this antibody with validated specificity and reproducibility for research into serotonergic neurotransmission, gastrointestinal physiology, and receptor pharmacology. By enabling detailed examination of 5-hydroxytryptamine receptor 3E expression and function, the HTR3E antibody advances understanding of serotonin receptor biology and its relevance to neural and digestive system disorders.

UniProt: A5X5Y0