SH2B1 antibody (AA 26-715)

Catalog No. ABIN7873014

The Rabbit Polyclonal anti-SH2B1 antibody is suitable to detect SH2B1 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for SH2B1 antibody (AA 26-715) (ABIN7873014)

Target: SH2B1 (SH2B Adaptor Protein 1 (SH2B1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SH2B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SH2B1 Antibody

Binding Specificity: AA 26-715

Purpose: SH2B1 Antibody / SH2B adapter protein 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SH2B1 recombinant protein (Position: W26-Q715) was used as the immunogen for the SH2B1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SH2B1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SH2B1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SH2B1

Target: SH2B1 (SH2B Adaptor Protein 1 (SH2B1))

Alternative Name: SH2B1

Background: SH2B1 antibody detects SH2B adapter protein 1, a signaling adaptor that regulates insulin, leptin, and growth factor receptor pathways controlling metabolism and energy balance. Encoded by the SH2B1 gene on chromosome 16p11.2, this cytoplasmic adaptor belongs to the SH2B family of signal transduction proteins and serves as a key modulator of receptor tyrosine kinase and cytokine receptor signaling. SH2B1 enhances insulin receptor signaling, JAK2 activation, and downstream PI3K and MAPK pathways involved in glucose metabolism, cell growth, and neuronal signaling.

Structurally, SH2B1 is a 756-amino-acid protein of approximately 85 kilodaltons containing an N-terminal dimerization domain, a central pleckstrin homology (PH) domain, and a C-terminal Src homology 2 (SH2) domain that mediates phosphotyrosine-dependent interactions. It exists in multiple isoforms (alpha, beta, gamma, and delta) produced by alternative splicing, with tissue-specific expression across brain, liver, adipose tissue, and skeletal muscle. The protein acts as a scaffold that bridges receptor kinases to intracellular effectors, amplifying or modulating signaling intensity and duration.

The SH2B1 antibody is widely used in metabolism, endocrinology, and neurobiology research to study insulin signaling, leptin sensitivity, and receptor-mediated pathways regulating energy homeostasis. Western blot analysis detects a band of approximately 85 kilodaltons corresponding to SH2B1, while immunofluorescence reveals cytoplasmic and plasma membrane localization. This antibody is an essential reagent for exploring the molecular basis of metabolic signaling and neuronal regulation.

Functionally, SH2B1 binds to insulin and leptin receptors through JAK2 and IRS1, enhancing phosphorylation cascades that regulate glucose uptake and appetite control. Loss or mutation of SH2B1 disrupts these pathways, leading to insulin resistance, obesity, and metabolic syndrome. Deletions encompassing SH2B1 are associated with severe early-onset obesity and neurobehavioral abnormalities, while overexpression can increase leptin sensitivity and improve glucose tolerance. Beyond metabolism, SH2B1 contributes to neuronal growth, regeneration, and synaptic plasticity through its modulation of NGF and BDNF signaling. The SH2B1 antibody provides a robust tool for studying these signaling mechanisms and their implications in metabolic and neurological disorders. NSJ Bioreagents validates this antibody for its applications, ensuring specific and reproducible results across biological systems.

UniProt: Q9NRF2