TPCN2 antibody (AA 26-752)

Catalog No. ABIN7873017

The Rabbit Polyclonal anti-TPCN2 antibody is suitable to detect TPCN2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and FACS.

Quick Overview for TPCN2 antibody (AA 26-752) (ABIN7873017)

Target: TPCN2 (Two Pore Segment Channel 2 (TPCN2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPCN2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TPCN2 Antibody

Binding Specificity: AA 26-752

Purpose: TPCN2 Antibody / Two-pore channel 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TPCN2 recombinant protein (Position: L26-R752) was used as the immunogen for the TPCN2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TPCN2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TPCN2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TPCN2

Target: TPCN2 (Two Pore Segment Channel 2 (TPCN2))

Alternative Name: TPCN2

Background: TPCN2 antibody detects Two-pore channel 2, an endolysosomal cation channel that mediates calcium and sodium flux in response to nicotinic acid adenine dinucleotide phosphate (NAADP) signaling. Encoded by the TPCN2 gene on chromosome 11q13.4, this channel is part of the two-pore channel family that regulates intracellular calcium release from acidic organelles such as lysosomes and endosomes. TPCN2 contains two homologous pore-forming domains, each with six transmembrane helices, and functions as a homodimeric voltage-gated ion channel. It plays a key role in vesicle trafficking, autophagy, endosomal fusion, and cellular excitability.

TPCN2 is widely expressed in kidney, liver, pancreas, and smooth muscle tissues, where it coordinates calcium-dependent signaling events. Activation of TPCN2 by NAADP triggers localized calcium release that modulates downstream pathways, including mTOR signaling, lysosomal exocytosis, and metabolic adaptation. Genetic studies have linked TPCN2 variants to pigmentation differences and hair color determination, highlighting its broader physiological influence. In neurons, TPCN2 regulates endolysosomal dynamics essential for synaptic vesicle recycling and signal transmission.

The TPCN2 antibody is a key reagent for cell signaling, membrane biology, and neurophysiology studies. Western blot analysis identifies a 75 kilodalton band corresponding to full-length TPCN2, while immunofluorescence reveals punctate staining consistent with lysosomal localization. The antibody enables detection of TPCN2 in subcellular fractionation and colocalization experiments with LAMP1 and Rab7 markers. Because endolysosomal calcium release impacts autophagy, metabolism, and exocytosis, this antibody supports research into pathways controlling intracellular trafficking and energy homeostasis.

Loss or dysregulation of TPCN2 impairs lysosomal function, alters autophagic flux, and contributes to disorders involving calcium signaling imbalance. Elevated expression has been associated with cancer cell invasiveness and altered vesicular pH homeostasis. The TPCN2 antibody allows researchers to evaluate how NAADP-dependent calcium signaling influences physiological and pathological processes. NSJ Bioreagents provides this antibody validated for its applications, ensuring accurate and reproducible detection of Two-pore channel 2 across species.

UniProt: Q8NHX9