GATA4 antibody (AA 262-321)

Catalog No. ABIN7873034

This Mouse Monoclonal antibody specifically detects GATA4 in FACS. It exhibits reactivity toward Human.

Quick Overview for GATA4 antibody (AA 262-321) (ABIN7873034)

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GATA4 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: PCRP-GATA4-1A7

Product Details anti-GATA4 Antibody

Binding Specificity: AA 262-321

Purpose: GATA4 Antibody / GATA-binding factor 4

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 262-321) from the human protein was used as the immunogen for the GATA4 antibody.

Isotype: IgG2b

Application Details

Application Notes: Optimal dilution of the GATA4 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot the GATA4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for GATA4

Target: GATA4 (GATA Binding Protein 4 (GATA4))

Alternative Name: GATA4

Background: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4, expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

UniProt: P43694

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis