Phospholipase C beta 2 antibody (AA 27-1185)

Catalog No. ABIN7873076

This Rabbit Polyclonal antibody specifically detects Phospholipase C beta 2 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for Phospholipase C beta 2 antibody (AA 27-1185) (ABIN7873076)

Target: Phospholipase C beta 2 (PLCb2) (phospholipase C beta 2 (PLCb2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C beta 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-Phospholipase C beta 2 Antibody

Binding Specificity: AA 27-1185

Purpose: PLCB2 Antibody / Phospholipase C-beta-2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PLCB2 recombinant protein (Position: D27-L1185) was used as the immunogen for the PLCB2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PLCB2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PLCB2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Phospholipase C beta 2

Target: Phospholipase C beta 2 (PLCb2) (phospholipase C beta 2 (PLCb2))

Alternative Name: PLCB2

Background: The PLCB2 antibody targets 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 (Phospholipase C-beta-2), an enzyme encoded by the PLCB2 gene. This beta isoform of phospholipase C hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) into the second messengers inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG). These products regulate calcium signaling and protein kinase C activation, forming a critical pathway downstream of G-protein-coupled receptors (GPCRs) and immune receptors. The PLCB2 antibody provides a specific tool for exploring phosphoinositide signaling, calcium homeostasis, and receptor-mediated cellular activation.

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 is widely expressed in hematopoietic and epithelial cells. It interacts with G alpha q and G beta gamma subunits of heterotrimeric G-proteins, linking receptor activation to intracellular calcium mobilization. The PLCB2 antibody enables detection of this enzyme in tissues where rapid signal transduction is required, such as immune cells and neurons. Its enzymatic activity is central to processes like secretion, chemotaxis, and proliferation.

PLCB2 plays a pivotal role in innate and adaptive immunity by coupling receptor engagement to downstream effector responses. The PLCB2 antibody supports investigations into Fc receptor signaling, platelet activation, and leukocyte migration. Dysregulation of phospholipase C beta-2 can lead to aberrant immune signaling, inflammation, and altered calcium dynamics that contribute to autoimmune or cardiovascular disorders.

In addition to its immune functions, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 contributes to sensory transduction in olfactory and gustatory pathways. The PLCB2 antibody is therefore valuable for neuroscience and sensory-biology research. Knockout models reveal its necessity in taste signaling and mucosal immune defense.

The PLCB2 antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, typically revealing cytoplasmic and membrane-associated staining. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for cell signaling, immunology, and biochemistry research. By enabling accurate detection of 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2, the PLCB2 antibody advances understanding of G-protein-coupled signaling networks and calcium-dependent cellular processes.

UniProt: Q00722

Pathways: WNT Signaling, Thyroid Hormone Synthesis, CXCR4-mediated Signaling Events, G-protein mediated Events, Thromboxane A2 Receptor Signaling