TMED10 antibody (AA 27-194)

Catalog No. ABIN7873092

The Rabbit Polyclonal anti-TMED10 antibody is suitable to detect TMED10 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC, IF and FACS.

Quick Overview for TMED10 antibody (AA 27-194) (ABIN7873092)

Target: TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TMED10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TMED10 Antibody

Binding Specificity: AA 27-194

Purpose: TMED10 Antibody / Transmembrane p24 trafficking protein 10

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TMP21/TMED10 recombinant protein (Position: R27-M194) was used as the immunogen for the TMED10 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TMED10 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TMED10 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TMED10

Target: TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))

Alternative Name: TMED10

Background: TMED10 antibody detects Transmembrane p24 trafficking protein 10, encoded by the TMED10 gene. Transmembrane p24 trafficking protein 10 is a member of the p24 family of cargo receptors that regulate vesicular trafficking between the endoplasmic reticulum and the Golgi apparatus. TMED10 antibody provides researchers with a reliable reagent to study vesicle transport, protein secretion, and the organization of the early secretory pathway.

Transmembrane p24 trafficking protein 10 is an integral membrane protein with a luminal GOLD domain, a transmembrane region, and a short cytoplasmic tail. Research using TMED10 antibody has shown that it interacts with coat protein complexes COPI and COPII, which mediate vesicle budding and fusion. These interactions allow TMED10 to function as a cargo receptor and regulator of vesicle biogenesis. Its role ensures that proteins are correctly sorted and transported between organelles.

Studies with TMED10 antibody have demonstrated that this protein is essential for the proper trafficking of glycosylphosphatidylinositol anchored proteins and other secretory cargo. Without TMED10, cargo retention and mislocalization occur, disrupting Golgi organization and secretion. This highlights the central importance of TMED10 in maintaining homeostasis of the secretory system.

Dysregulation of Transmembrane p24 trafficking protein 10 has been associated with cancer and neurodegeneration. Research using TMED10 antibody has shown that altered expression modifies the trafficking of growth factor receptors and adhesion molecules, promoting abnormal signaling. Variants in TMED10 have also been linked to altered susceptibility to viral infection, emphasizing its role in cellular defense. These findings underscore its broad physiological and pathological relevance.

TMED10 antibody is commonly used in immunohistochemistry, immunofluorescence, and western blotting. Immunohistochemistry reveals distribution in tissues with active secretion, immunofluorescence highlights Golgi localization, and western blotting quantifies expression levels. These applications make TMED10 antibody a valuable tool for cell biology research.

By providing validated TMED10 antibody reagents, NSJ Bioreagents supports studies into vesicle trafficking, protein secretion, and disease. Detection of Transmembrane p24 trafficking protein 10 provides researchers with insights into how the p24 family of proteins coordinates early secretory pathways.

UniProt: P49755