IFI30 antibody (AA 27-250)

Catalog No. ABIN7873105

The Rabbit Polyclonal anti-IFI30 antibody (ABIN7873105) specifically detects IFI30 in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for IFI30 antibody (AA 27-250) (ABIN7873105)

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFI30 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-IFI30 Antibody

Binding Specificity: AA 27-250

Purpose: IFI30 Antibody / GILT / Gamma-interferon-inducible lysosomal thiol reductase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human IFI30 recombinant protein (Position: S27-K250) was used as the immunogen for the IFI30 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the IFI30 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the IFI30 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for IFI30

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Alternative Name: IFI30

Background: IFI30 antibody detects Gamma-interferon-inducible lysosomal thiol reductase, an enzyme that catalyzes the reduction of disulfide bonds in endosomal and lysosomal compartments. The UniProt recommended name is Gamma-interferon-inducible lysosomal thiol reductase (IFI30). This enzyme facilitates antigen processing by reducing disulfide-linked epitopes within endosomes, promoting peptide loading onto MHC class II molecules for immune presentation.

Functionally, IFI30 antibody identifies a 250-amino-acid glycoprotein localized to lysosomes and late endosomes. IFI30 acts in concert with gamma-interferon-induced pathways to maintain redox balance within antigen-presenting cells. Its active site cysteines participate in thiol-disulfide exchange reactions, regenerating reduced peptides that can be efficiently bound by MHC class II for recognition by CD4+ T cells.

The IFI30 gene is located on chromosome 19p13.1 and is expressed predominantly in macrophages, dendritic cells, and B lymphocytes. Its expression is upregulated by interferon-gamma signaling and inflammatory cytokines. IFI30 functions downstream of antigen uptake and proteolysis, serving as a critical component in adaptive immune activation and tolerance maintenance.

Pathologically, altered IFI30 activity has been linked to immune disorders and cancer. Overexpression can enhance antigen presentation and immune surveillance, while deficiencies impair immune responses and tolerance mechanisms. IFI30 is also explored as a marker of macrophage activation and tumor immunogenicity. Research using IFI30 antibody supports immunology, antigen processing, and redox regulation studies.

IFI30 antibody is suitable for western blotting, immunohistochemistry, and immunofluorescence to detect lysosomal enzymes and immune regulators. NSJ Bioreagents provides validated IFI30 antibody reagents for use in immunology, cell biology, and redox regulation research.

Structurally, Gamma-interferon-inducible lysosomal thiol reductase contains a thioredoxin-like domain and a signal peptide targeting it to lysosomes. The protein undergoes proteolytic maturation to its active form within acidic compartments. This antibody facilitates the study of IFI30's enzymatic and immunomodulatory roles in antigen processing and cellular redox maintenance.

UniProt: P13284