TFEC antibody (AA 27-311)

Catalog No. ABIN7873111

The Rabbit Polyclonal anti-TFEC antibody (ABIN7873111) specifically detects TFEC in ELISA, WB, ICC, IF and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for TFEC antibody (AA 27-311) (ABIN7873111)

Target: TFEC (Transcription Factor EC (TFEC))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFEC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-TFEC Antibody

Binding Specificity: AA 27-311

Purpose: TFEC Antibody / Transcription factor EC

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TFEC recombinant protein (Position: Q27-D311) was used as the immunogen for the TFEC antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TFEC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TFEC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TFEC

Target: TFEC (Transcription Factor EC (TFEC))

Alternative Name: TFEC

Background: TFEC antibody detects Transcription factor EC, a basic helix-loop-helix leucine zipper (bHLH-LZ) transcription factor that regulates gene expression in macrophages, osteoclasts, and melanocytes. TFEC belongs to the MiT/TFE family, which includes MITF, TFE3, and TFEB, all of which coordinate transcriptional responses to nutrient availability, lysosomal function, and immune signaling. The TFEC antibody is widely used in transcriptional regulation, macrophage differentiation, and cancer research.

TFEC is encoded by the TFEC gene located on human chromosome 7q31.2. The protein is approximately 38 kilodaltons and contains a conserved DNA-binding bHLH-LZ domain that mediates binding to E-box sequences in gene promoters. TFEC forms homodimers or heterodimers with other MiT family members, allowing versatile regulation of target gene networks. It localizes to the nucleus and is activated by phosphorylation under stress or inflammatory conditions.

The TFEC antibody detects a 38 kilodalton protein by western blot and demonstrates nuclear staining in macrophages and osteoclast precursors. TFEC regulates transcription of lysosomal and autophagy-related genes, participating in cellular adaptation to nutrient deprivation. It also modulates inflammatory gene expression through cooperation with PU.1 and other macrophage-specific transcription factors, promoting cytokine and chemokine production.

In melanocytes, TFEC contributes to pigment cell differentiation and interacts with MITF to balance melanogenic gene expression. Dysregulation of TFEC has been implicated in cancer, where fusion genes involving TFEC drive aberrant transcriptional programs. Altered expression is also associated with inflammatory and metabolic disorders due to disrupted lysosomal signaling and autophagic control.

TFEC integrates environmental and metabolic signals to maintain homeostasis in immune and pigment cells. NSJ Bioreagents provides a validated TFEC antibody optimized for western blot, immunofluorescence, and transcriptional studies, supporting detailed analysis of gene regulation, macrophage biology, and MiT/TFE family signaling.

UniProt: O14948