SPIDR antibody (AA 273-904)

Catalog No. ABIN7873197

The Rabbit Polyclonal anti-SPIDR antibody is suitable to detect SPIDR in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS, ICC and IF.

Quick Overview for SPIDR antibody (AA 273-904) (ABIN7873197)

Target: SPIDR (Scaffolding Protein Involved in DNA Repair (SPIDR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPIDR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-SPIDR Antibody

Binding Specificity: AA 273-904

Purpose: SPIDR Antibody / Scaffold protein involved in DNA repair

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SPIDR recombinant protein (Position: E273-E904) was used as the immunogen for the SPIDR antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SPIDR antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SPIDR antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPIDR

Target: SPIDR (Scaffolding Protein Involved in DNA Repair (SPIDR))

Alternative Name: SPIDR

Background: SPIDR antibody detects Scaffold protein involved in DNA repair (also known as KIAA0146), a nuclear adaptor protein that coordinates homologous recombination and DNA double-strand break repair. Encoded by the SPIDR gene on chromosome 8q24.3, this protein functions as a scaffold linking key repair enzymes including RAD51, BRCA2, and RPA to the DNA damage response machinery. SPIDR facilitates the assembly of repair complexes at damaged DNA sites and promotes strand invasion and exchange during homologous recombination.

SPIDR contains multiple interaction domains, including coiled-coil regions and binding motifs that mediate association with RAD51 paralogs and BRCA2. Following DNA damage, SPIDR localizes to nuclear foci corresponding to double-strand break sites, where it promotes RAD51 filament formation and repair fidelity. By stabilizing repair intermediates, SPIDR ensures proper resolution of recombination events and maintenance of genomic stability. Depletion of SPIDR impairs DNA repair efficiency and increases chromosomal aberrations, underscoring its importance in genome maintenance.

The SPIDR antibody is used in DNA repair, cancer, and cell cycle research to analyze homologous recombination pathways and genomic stability. Western blot analysis identifies a 90 kilodalton band corresponding to SPIDR, while immunofluorescence shows punctate nuclear foci following genotoxic stress. This antibody enables researchers to track DNA repair factor recruitment and assess homologous recombination efficiency.

Defects in SPIDR expression or localization are associated with hypersensitivity to DNA-damaging agents and increased genomic instability, contributing to cancer susceptibility. SPIDR also cooperates with checkpoint proteins such as ATR and CHK1 to coordinate DNA repair with replication stress responses. The SPIDR antibody supports these investigations by providing specific detection in western blotting, flow cytometry, and immunofluorescence applications. NSJ Bioreagents validates this antibody for use in research on DNA repair dynamics, chromatin remodeling, and tumor suppressor pathways.

UniProt: Q14159

Pathways: Positive Regulation of Response to DNA Damage Stimulus