YBX1 antibody (AA 275-310)

Catalog No. ABIN7873204

The Rabbit Polyclonal anti-YBX1 antibody (ABIN7873204) specifically detects YBX1 in WB. The antibody is reactive with Zebrafish (Danio rerio) samples.

Quick Overview for YBX1 antibody (AA 275-310) (ABIN7873204)

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Reactivity: Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This YBX1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-YBX1 Antibody

Binding Specificity: AA 275-310

Purpose: Zebrafish Yb-1 Antibody / Ybx1 / Y-box-binding protein 1

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived zebrafish Yb-1 recombinant protein (amino acids R275-D310) was used as the immunogen for the Zebrafish Yb-1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal dilution of the Zebrafish Yb-1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Zebrafish Yb-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for YBX1

Target: YBX1 (Y Box Binding Protein 1 (YBX1))

Alternative Name: Yb-1

Background: Y-box binding protein 1 (Ybx1, Yb-1) is a multifunctional RNA- and DNA-binding protein conserved across vertebrates. In zebrafish, Yb-1 plays a critical role in early embryogenesis, cellular proliferation, and gene regulation. The protein contains a conserved cold shock domain (CSD) that facilitates binding to nucleic acids, enabling Yb-1 to modulate transcription, mRNA splicing, stability, and translation.

Biological Function:
Yb-1 is essential for zebrafish embryonic development. Knockdown or mutation of Yb-1 results in early embryonic lethality due to impaired morphogenesis, suggesting a key regulatory role in zygotic gene activation and maternal RNA clearance. It is also involved in stress responses, regulation of cell cycle genes, and may contribute to sex determination pathways in zebrafish.

Expression Pattern:
Yb-1 is maternally deposited and ubiquitously expressed during early embryonic stages. As development progresses, its expression becomes more tissue-specific, particularly enriched in proliferative zones and neural tissues.

Orthology and Conservation:
Zebrafish Ybx1 is orthologous to human YBX1, sharing high sequence similarity and functional conservation. This makes zebrafish a valuable model for studying YBX1-related roles in development and disease, including cancer biology and RNA regulation.

UniProt: B5DE31

Pathways: Regulation of Muscle Cell Differentiation