PDZD8 antibody (AA 28-1146)

Catalog No. ABIN7873225

The Rabbit Polyclonal anti-PDZD8 antibody (ABIN7873225) specifically detects PDZD8 in WB, ELISA, FACS, IF and IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for PDZD8 antibody (AA 28-1146) (ABIN7873225)

Target: PDZD8 (PDZ Domain Containing 8 (PDZD8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDZD8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-PDZD8 Antibody

Binding Specificity: AA 28-1146

Purpose: PDZD8 Antibody / PDZ domain-containing protein 8

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PDZD8 recombinant protein (Position: R28-D1146) was used as the immunogen for the PDZD8 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PDZD8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PDZD8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PDZD8

Target: PDZD8 (PDZ Domain Containing 8 (PDZD8))

Alternative Name: PDZD8

Background: PDZD8 antibody targets PDZ domain-containing protein 8, a membrane-associated scaffolding protein involved in organelle tethering, lipid transfer, and calcium signaling. Encoded by the PDZD8 gene, this multidomain protein contains PDZ, C1, and coiled-coil regions that mediate interactions with multiple organelle and cytoskeletal proteins. PDZD8 plays a crucial role in forming membrane contact sites between the endoplasmic reticulum (ER) and mitochondria, facilitating lipid exchange and calcium ion transport essential for energy metabolism and cellular homeostasis.

Recent studies have shown that PDZD8 acts as a functional ortholog of yeast Mmm1-Mdm12 complex components in establishing ER-mitochondrial contacts. By bridging the two organelles, PDZD8 helps coordinate mitochondrial dynamics and calcium uptake during neuronal signaling and metabolic activity. Loss of PDZD8 disrupts mitochondrial morphology, impairs synaptic transmission, and affects neuronal energy balance, highlighting its importance in brain physiology. The protein is also implicated in autophagy regulation, as it contributes to the spatial organization of membrane contact sites during autophagosome formation.

The PDZD8 gene is located on chromosome 10q25.3 and encodes multiple isoforms through alternative splicing. The protein localizes to the ER membrane via its C-terminal transmembrane domain and interacts with small GTPases and lipid transport proteins. Dysregulation of PDZD8 has been associated with neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS) and Huntington's disease, where altered organelle tethering and calcium imbalance contribute to pathogenesis.

Immunohistochemical staining using PDZD8 antibody reveals perinuclear and cytoplasmic localization in neurons, hepatocytes, and fibroblasts. The antibody is valuable for investigating ER-mitochondrial contact formation, calcium signaling, and membrane lipid metabolism. PDZD8 antibody from NSJ Bioreagents supports research into cellular organelle communication, neurodegenerative mechanisms, and intracellular trafficking.

UniProt: Q8NEN9