GRK1 antibody (AA 28-524)

Catalog No. ABIN7873272

The Rabbit Polyclonal anti-GRK1 antibody is suitable to detect GRK1 in samples from Mouse and Rat. It has been validated for WB, IHC and ELISA.

Quick Overview for GRK1 antibody (AA 28-524) (ABIN7873272)

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-GRK1 Antibody

Binding Specificity: AA 28-524

Purpose: GRK1 Antibody / G protein-coupled receptor kinase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GRK1 recombinant protein (Position: Q28-E524) was used as the immunogen for the GRK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GRK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GRK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GRK1

Target: GRK1 (G Protein-Coupled Receptor Kinase 1 (GRK1))

Alternative Name: GRK1

Background: GRK1 antibody detects G protein-coupled receptor kinase 1, a serine/threonine kinase responsible for phosphorylating light-activated rhodopsin in photoreceptor cells. The UniProt recommended name is G protein-coupled receptor kinase 1 (GRK1). Also known as Rhodopsin kinase, this enzyme plays a vital role in visual signal termination and photoreceptor recovery following light stimulation.

Functionally, GRK1 antibody identifies a 563-amino-acid cytoplasmic enzyme containing an N-terminal regulator of G protein signaling (RGS) domain, a central catalytic kinase domain, and a C-terminal prenylation site that targets it to photoreceptor membranes. GRK1 phosphorylates activated rhodopsin, facilitating its binding to arrestin and preventing further activation of transducin, thus restoring photoreceptor sensitivity.

The GRK1 gene is located on chromosome 13q34 and is specifically expressed in retinal rod cells and pinealocytes. GRK1i 1/2s activity is essential for proper phototransduction and dark adaptation. Its regulation ensures rapid recovery of vision after exposure to light and protects photoreceptors from overstimulation.

Pathologically, GRK1 mutations cause Oguchi disease type 2 and congenital stationary night blindness due to impaired rhodopsin deactivation. Dysregulated GRK1 activity can also contribute to retinal degeneration through prolonged receptor activation and phototoxicity. Research using GRK1 antibody supports studies in phototransduction, GPCR signaling, and inherited retinal disorders.

GRK1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect visual cycle kinases and photoreceptor signaling proteins. NSJ Bioreagents provides GRK1 antibody reagents optimized for vision research, GPCR regulation, and neurobiology studies.

Structurally, G protein-coupled receptor kinase 1 belongs to the AGC kinase family, with catalytic residues coordinating ATP and substrate binding. Its C-terminal prenylation motif facilitates membrane association. This antibody aids investigation of GRK1's role in receptor desensitization and visual adaptation.

UniProt: Q15835

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction