TTBK1 antibody (AA 285-956)

Catalog No. ABIN7873316

This Rabbit Polyclonal antibody specifically detects TTBK1 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TTBK1 antibody (AA 285-956) (ABIN7873316)

Target: TTBK1 (tau Tubulin Kinase 1 (TTBK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTBK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-TTBK1 Antibody

Binding Specificity: AA 285-956

Purpose: TTBK1 Antibody / Tau-tubulin kinase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TTBK1 recombinant protein (Position: D285-A956) was used as the immunogen for the TTBK1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TTBK1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TTBK1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TTBK1

Target: TTBK1 (tau Tubulin Kinase 1 (TTBK1))

Alternative Name: TTBK1

Background: TTBK1 antibody detects Tau-tubulin kinase 1, a serine/threonine kinase involved in microtubule regulation, neuronal polarization, and tau protein phosphorylation. The UniProt recommended name is Tau-tubulin kinase 1 (TTBK1). This neuron-specific kinase phosphorylates tau and tubulin to modulate microtubule dynamics and axonal stability, playing key roles in neurodevelopment and neurodegenerative disease.

Functionally, TTBK1 antibody identifies a 1,326-amino-acid cytoplasmic and axonal kinase that phosphorylates tau at multiple sites, including those associated with Alzheimer's disease pathology. TTBK1 regulates microtubule nucleation and cilia disassembly, contributing to neuronal morphogenesis and signaling. It is primarily expressed in the cerebral cortex, hippocampus, and cerebellum, where it modulates cytoskeletal organization and synaptic plasticity.

The TTBK1 gene is located on chromosome 6p21.1 and is expressed predominantly in neurons. Expression is upregulated during axonal outgrowth and differentiation, linking kinase activity to neural development. TTBK1 functions within signaling cascades involving CDK5 and GSK3beta, coordinating tau modification and cytoskeletal remodeling in neurons.

Pathologically, aberrant activation or overexpression of TTBK1 contributes to tau hyperphosphorylation, neurofibrillary tangle formation, and neuronal death in Alzheimer's disease and other tauopathies. Mutations or altered regulation may also affect primary cilia dynamics and neuronal polarity. Research using TTBK1 antibody supports studies in neurodegeneration, kinase signaling, and cytoskeletal biology.

TTBK1 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect neuronal kinases. NSJ Bioreagents provides TTBK1 antibody reagents optimized for research in tau phosphorylation, axonal transport, and neurodegenerative mechanisms.

Structurally, Tau-tubulin kinase 1 contains an N-terminal kinase domain with conserved ATP-binding and catalytic residues, and a C-terminal regulatory region that mediates substrate specificity and microtubule association. TTBK1 undergoes autophosphorylation and dynamic localization between cytoplasm and axon terminals. This antibody enables detailed examination of TTBK1's role in neuronal architecture and tau-related pathology.

UniProt: Q5TCY1