Butyrylcholinesterase antibody (AA 29-602)

Catalog No. ABIN7873434

The Rabbit Polyclonal anti-Butyrylcholinesterase antibody is suitable to detect Butyrylcholinesterase in samples from Human. It has been validated for WB and ELISA.

Quick Overview for Butyrylcholinesterase antibody (AA 29-602) (ABIN7873434)

Target: Butyrylcholinesterase (BCHE)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Butyrylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Butyrylcholinesterase Antibody

Binding Specificity: AA 29-602

Purpose: BCHE Antibody / Butyrylcholinesterase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human BCHE recombinant protein (Position: E29-L602) was used as the immunogen for the BCHE antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the BCHE antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the BCHE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Butyrylcholinesterase

Target: Butyrylcholinesterase (BCHE)

Alternative Name: BCHE

Background: BCHE antibody detects Butyrylcholinesterase, a serine hydrolase that catalyzes the hydrolysis of esters of choline, contributing to neurotransmitter metabolism and detoxification of xenobiotics. The UniProt recommended name is Butyrylcholinesterase (BCHE). This enzyme is found predominantly in plasma, liver, and nervous tissue and acts as a metabolic complement to acetylcholinesterase in cholinergic regulation.

Functionally, BCHE antibody identifies a 602-amino-acid glycoprotein secreted as a tetrameric enzyme that hydrolyzes choline esters such as butyrylcholine and succinylcholine. BCHE regulates cholinergic neurotransmission indirectly by hydrolyzing circulating esters and serves as a bioscavenger for organophosphorus compounds and nerve agents. It also participates in drug metabolism by inactivating esterified anesthetics and muscle relaxants.

The BCHE gene is located on chromosome 3q26.1-q26.2 and encodes a protein synthesized mainly in the liver before secretion into plasma. BCHE shares structural homology with acetylcholinesterase but differs in substrate specificity and inhibitor sensitivity. Its activity is regulated by genetic polymorphisms that influence catalytic efficiency and drug sensitivity. Reduced BCHE activity causes prolonged neuromuscular blockade following exposure to succinylcholine or mivacurium, a clinically relevant pharmacogenetic condition.

Beyond neurotransmission, BCHE plays roles in lipid metabolism, inflammation, and neurodegenerative diseases. Elevated BCHE activity is associated with obesity and metabolic syndrome, while decreased activity has been observed in liver dysfunction, Alzheimer's disease, and chronic inflammation. Its ability to hydrolyze ghrelin and other acylated hormones links it to appetite regulation and energy balance. In neurobiology, BCHE is thought to modulate cholinergic tone during aging and cognitive decline.

BCHE antibody is widely used in neurobiology, pharmacology, and metabolic disease research. It is suitable for western blotting, ELISA, and enzyme histochemistry to detect BCHE in plasma, liver, or neural tissue. This antibody supports studies of cholinesterase activity, drug metabolism, and neurodegenerative mechanisms. In toxicology, BCHE detection helps evaluate exposure to organophosphate inhibitors and nerve agents.

Structurally, BCHE is a glycoprotein composed of four identical subunits stabilized by disulfide bonds and carbohydrate moieties. Its catalytic triad (Ser-His-Glu) mediates ester hydrolysis through a charge-relay mechanism. NSJ Bioreagents provides BCHE antibody reagents validated for use in neurotransmission, toxicology, and enzyme metabolism research.

UniProt: P06276

Pathways: Peptide Hormone Metabolism