ITPKC antibody (AA 292-607)

Catalog No. ABIN7873450

The Rabbit Polyclonal anti-ITPKC antibody (ABIN7873450) specifically detects ITPKC in WB, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for ITPKC antibody (AA 292-607) (ABIN7873450)

Target: ITPKC (Inositol-Trisphosphate 3-Kinase C (ITPKC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ITPKC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-ITPKC Antibody

Binding Specificity: AA 292-607

Purpose: ITPKC Antibody / Inositol-trisphosphate 3-kinase C

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ITPKC recombinant protein (Position: D292-E607) was used as the immunogen for the ITPKC antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ITPKC antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ITPKC antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ITPKC

Target: ITPKC (Inositol-Trisphosphate 3-Kinase C (ITPKC))

Alternative Name: ITPKC

Background: ITPKC antibody detects Inositol-trisphosphate 3-kinase C, a signaling enzyme that phosphorylates inositol 1,4,5-trisphosphate (IP3) to generate inositol 1,3,4,5-tetrakisphosphate (IP4). ITPKC serves as a critical regulator of intracellular calcium signaling, immune activation, and vascular inflammation. The ITPKC antibody is widely used in immunology, signal transduction, and cardiovascular research to study calcium homeostasis and immune cell regulation.

ITPKC is encoded by the ITPKC gene located on human chromosome 19q13.2. The protein is approximately 461 amino acids long and localizes to the cytoplasm, where it modulates calcium flux by regulating IP3 metabolism. Through phosphorylation of IP3, ITPKC reduces calcium release from the endoplasmic reticulum, thereby attenuating NFAT activation and T-cell proliferation.

The ITPKC antibody detects a 52 kilodalton band by western blot and exhibits diffuse cytoplasmic staining under fluorescence microscopy. ITPKC plays a pivotal role in immune regulation, particularly in T-cell receptor signaling. Genetic variants of ITPKC are associated with Kawasaki disease, an inflammatory vascular disorder, due to dysregulated calcium-dependent immune activation.

Beyond immunity, ITPKC influences endothelial barrier function and smooth muscle contraction, linking it to cardiovascular homeostasis. It also interacts with other kinases and phosphatases in the phosphoinositide signaling pathway, integrating metabolic and signaling cues. In cancer cells, ITPKC expression affects proliferation and angiogenesis by controlling intracellular calcium oscillations.

Because of its dual role in immune and vascular signaling, ITPKC is a key enzyme at the crossroads of inflammation and calcium dynamics. NSJ Bioreagents provides a validated ITPKC antibody optimized for its applications, supporting research into immune regulation, vascular biology, and phosphoinositide metabolism.

UniProt: Q96DU7