LAG3 antibody (AA 300-500)

Catalog No. ABIN7873639

The Mouse Monoclonal anti-LAG3 antibody (Clone LAG3-7380) (ABIN7873639) specifically detects LAG3 in IHC (p). The antibody is reactive with Human samples.

Quick Overview for LAG3 antibody (AA 300-500) (ABIN7873639)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: LAG3-7380

Product Details anti-LAG3 Antibody

Binding Specificity: AA 300-500

Purpose: Lymphocyte activation gene 3 Antibody / LAG3 / CD223

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 300-500) from the human protein was used as the immunogen for the Lymphocyte activation gene 3 antibody.

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal dilution of the Lymphocyte activation gene 3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Lymphocyte activation gene 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: Lymphocyte activation gene 3

Background: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4+CD8+ T cells and NK cell, with shared homology in structure to CD4 Molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3+CD4+CD8+ T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved KIEELE motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3+ cells) and inhibit induced (i.e. antigen-specific) Treg suppressive activity.

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints