Nidogen 1 antibody (AA 31-1209)

Catalog No. ABIN7873746

The Rabbit Polyclonal anti-Nidogen 1 antibody is suitable to detect Nidogen 1 in samples from Mouse and Human. It has been validated for ELISA, WB, IHC, IF and FACS.

Quick Overview for Nidogen 1 antibody (AA 31-1209) (ABIN7873746)

Target: Nidogen 1 (NID1)

Reactivity: Mouse, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nidogen 1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-Nidogen 1 Antibody

Binding Specificity: AA 31-1209

Purpose: NID1 Antibody / Nidogen 1 / Entactin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Entactin/NID1 recombinant protein (Position: R31-H1209) was used as the immunogen for the NID1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NID1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NID1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Nidogen 1

Target: Nidogen 1 (NID1)

Alternative Name: NID1

Background: NID1 antibody is used to study Nidogen 1, also known as Entactin, a key basement membrane linker that bridges laminin networks with collagen IV scaffolds to establish tissue architecture and mechanical stability. As a secreted glycoprotein with multiple binding modules, Nidogen 1 connects laminin gamma chains to collagen IV and perlecan, organizing a resilient sheet that supports epithelial polarity, filtration barriers, and neuromuscular junctions. Through these interactions, the protein shapes morphogenesis, angiogenesis, and repair, and it helps buffer tissues against mechanical stress.

Nidogen 1 contains globular domains separated by flexible segments that adapt to different extracellular partners. Its localization along the basement membrane enables crosstalk between matrix and cells, influencing receptor signaling through integrins and dystroglycan. Depletion in animal models disrupts organogenesis and compromises barrier function in kidney and vasculature, highlighting the protein's structural and signaling roles. Because matrix composition tunes growth factor availability, Nidogen 1 also contributes to gradient formation and ligand presentation within tissue microenvironments.

Researchers use NID1 antibody / Entactin in immunohistochemistry to delineate basement membranes in skin, kidney, lung, and tumor samples, providing clear borders for morphometric analyses and pathology scoring. In immunoblotting, the reagent tracks expression during development or matrix remodeling, while immunofluorescence co-localization with laminin and collagen IV reveals network integrity and continuity. In vitro, combining the antibody with matrix assembly assays clarifies how Nidogen 1 scaffolds influence cell adhesion, migration, and polarity under defined conditions.

The NID1 antibody / Entactin from NSJ Bioreagents performs reliably in tissue sections and cultured cells, enabling teams to standardize basement membrane visualization across experiments and sample types. By pairing matrix mapping with transcriptomics or proteomics, investigators can connect changes in Nidogen 1 to shifts in epithelial organization, vascular stability, and tumor-stroma interplay, thereby linking structural cues to functional outcomes in both physiology and disease.

UniProt: P14543