DKK2 antibody (AA 31-62)

Catalog No. ABIN7873799

This Rabbit Polyclonal antibody specifically detects DKK2 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for DKK2 antibody (AA 31-62) (ABIN7873799)

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DKK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-DKK2 Antibody

Binding Specificity: AA 31-62

Purpose: Dickkopf-2 Antibody / DKK2

Purification: Purified

Immunogen: A portion of amino acids 31-62 from the human protein was used as the immunogen for the Dickkopf-2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the Dickkopf-2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Dickkopf-2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for DKK2

Target: DKK2 (Dickkopf 2 Homolog (DKK2))

Alternative Name: Dickkopf-2

Background: The 259-amino acid DKK2 protein, like DKK1, DKK3, and DKK4, possesses an N-terminal signal peptide and 2 conserved cysteine-rich domains, which are separated by a linker region and contain 10 cys residues each. The second cys region has a putative lipid-binding function that may facilitate WNT/DKK interactions at the plasma membrane. The linker region contains 50 to 55 amino acids in DKK1, DKK2, and DKK4, whereas in DKK3 it contains only 12 amino acids. All DKKs have several potential sites for cleavage by furin-type proteases. Northern blot analysis revealed expression of 4.0- and 4.5-kb DKK2 transcripts in heart, brain, skeletal muscle, and lung. Western blot analysis showed that DKK2 is secreted as a 15- to 17-kD protein. Functional analysis determined that DKK2 does not block Xenopus Wnt8 induction of a secondary axis in frog embryos.

UniProt: Q9UBU2

Pathways: WNT Signaling