ACP2 antibody (AA 31-88)

Catalog No. ABIN7873805

This Rabbit Polyclonal antibody specifically detects ACP2 in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for ACP2 antibody (AA 31-88) (ABIN7873805)

Target: ACP2 (Acid Phosphatase 2, Lysosomal (ACP2))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ACP2 Antibody

Binding Specificity: AA 31-88

Purpose: ACP2 Antibody / Lysosomal acid phosphatase 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human ACP2 recombinant protein (Position: R31-H88) was used as the immunogen for the ACP2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the ACP2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the ACP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for ACP2

Target: ACP2 (Acid Phosphatase 2, Lysosomal (ACP2))

Alternative Name: ACP2

Background: ACP2 antibody detects Lysosomal acid phosphatase 2, an enzyme responsible for the hydrolysis of phosphate esters within the acidic environment of lysosomes. ACP2 plays an essential role in the degradation of macromolecules, phosphate turnover, and lysosomal homeostasis. It functions as part of a larger lysosomal complex that regulates both nutrient recycling and intracellular signaling. The ACP2 antibody is widely used in cell biology, metabolism, and pathology research to study lysosomal function, enzyme regulation, and metabolic disorders.

ACP2 is encoded by the ACP2 gene located on human chromosome 11p11.2. The protein is approximately 452 amino acids in length and is synthesized as a precursor that undergoes proteolytic cleavage into two subunitsi 1/2a 36 kilodalton alpha subunit and a 14 kilodalton beta subuniti 1/2that together form the active enzyme. ACP2 localizes predominantly to the lysosomal lumen but can also be detected in late endosomes and phagosomes, reflecting its involvement in diverse degradative pathways.

The ACP2 antibody detects the 50 kilodalton precursor and its processed subunits by western blot and shows punctate lysosomal staining by immunofluorescence microscopy. ACP2 participates in the hydrolysis of phosphate-containing substrates derived from nucleotides, phospholipids, and other macromolecules, releasing inorganic phosphate for metabolic reuse. It complements the function of acid phosphatase ACP3, ensuring redundancy in phosphate metabolism and cellular cleanup.

Mutations or deficiencies in ACP2 lead to acid phosphatase deficiency, a rare lysosomal storage disorder characterized by abnormal accumulation of phosphomonoesters and progressive neurodegeneration. Reduced ACP2 activity has also been implicated in osteopetrosis-like bone disorders and altered immune responses, as lysosomal enzymes regulate antigen presentation and macrophage function. Conversely, upregulation of ACP2 is observed during macrophage activation and tissue remodeling, highlighting its adaptive role in immune and metabolic balance.

As a lysosomal hydrolase linking phosphate metabolism to intracellular clearance, ACP2 is a valuable marker for lysosome integrity and metabolic health. NSJ Bioreagents provides a validated ACP2 antibody optimized for its applications, supporting research into catabolic pathways, phosphate metabolism, and lysosomal storage diseases.

UniProt: P11117