ATG4C antibody (AA 33-62)

Catalog No. ABIN7874009

This Rabbit Polyclonal antibody specifically detects ATG4C in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for ATG4C antibody (AA 33-62) (ABIN7874009)

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG4C antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATG4C Antibody

Binding Specificity: AA 33-62

Purpose: ATG4C Antibody / APG4C

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 33-62 from the human protein was used as the immunogen for the ATG4C antibody.

Isotype: Ig Fraction

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the ATG4C antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot the ATG4C antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for ATG4C

Target: ATG4C (Autophagy related 4C Cysteine Peptidase (ATG4C))

Alternative Name: ATG4C

Background: Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4 is a cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes.

UniProt: Q96DT6

Pathways: Autophagy