CPT2 antibody (AA 33-646)

Catalog No. ABIN7874010

The Rabbit Polyclonal anti-CPT2 antibody is suitable to detect CPT2 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, FACS and IHC (p).

Quick Overview for CPT2 antibody (AA 33-646) (ABIN7874010)

Target: CPT2 (Carnitine Palmitoyltransferase 2 (CPT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPT2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CPT2 Antibody

Binding Specificity: AA 33-646

Purpose: CPT2 Antibody / Carnitine O-palmitoyltransferase 2

Purification: Antigen Affinity purified

Immunogen: Amino acids Q33-D646 from the human protein were used as the immunogen for the CPT2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CPT2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CPT2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CPT2

Target: CPT2 (Carnitine Palmitoyltransferase 2 (CPT2))

Alternative Name: CPT2

Background: CPT2 antibody is a widely used reagent for studying fatty acid metabolism, mitochondrial function, and energy homeostasis. The encoded protein, carnitine O-palmitoyltransferase 2 (CPT2), is a key enzyme in the carnitine shuttle system that transports long-chain fatty acids into mitochondria for beta-oxidation. Located on the inner mitochondrial membrane, CPT2 catalyzes the conversion of acylcarnitine back to acyl-CoA, enabling fatty acids to undergo oxidation and generate ATP. This function makes CPT2 essential for energy production, particularly during fasting, exercise, and other metabolic stresses.

CPT2 operates in close coordination with CPT1, which resides on the outer mitochondrial membrane, and carnitine-acylcarnitine translocase (CACT), which shuttles acylcarnitines across the inner membrane. Together, these enzymes form a highly regulated system ensuring the efficient use of fatty acids as fuel. By facilitating long-chain fatty acid oxidation, CPT2 contributes to metabolic flexibility, energy balance, and cellular survival under nutrient-limited conditions.

Genetic defects in the CPT2 gene are the cause of carnitine palmitoyltransferase II deficiency, a rare inherited metabolic disorder. Clinical presentations include lethal neonatal forms, severe infantile hepatocardiomuscular disease, and the most common myopathic form characterized by recurrent episodes of muscle weakness, rhabdomyolysis, and myoglobinuria. These conditions highlight the indispensable role of CPT2 in energy metabolism and underscore its clinical significance. Mutations affecting CPT2 disrupt fatty acid oxidation, leading to impaired ATP production and accumulation of toxic lipid intermediates.

In addition to its metabolic importance, CPT2 has been implicated in broader biological processes, including thermogenesis and regulation of lipid signaling pathways. Research has also linked altered CPT2 activity to metabolic syndromes, insulin resistance, and susceptibility to muscle-related disorders. Its central role in lipid utilization makes CPT2 a critical subject of investigation in both basic and translational research.

The CPT2 antibody is widely applied in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to measure protein expression, localization, and regulation. These applications are useful for assessing mitochondrial metabolism, characterizing genetic deficiencies, and exploring therapeutic approaches for metabolic disease. For researchers focused on lipid metabolism, muscle biology, or mitochondrial disorders, the CPT2 antibody provides a reliable detection tool. NSJ Bioreagents offers validated antibodies that ensure reproducibility and accuracy in advanced molecular studies.

UniProt: P23786

Pathways: Regulation of Lipid Metabolism by PPARalpha, Monocarboxylic Acid Catabolic Process