RS1 antibody (AA 34-224)

Catalog No. ABIN7874065

The Rabbit Polyclonal anti-RS1 antibody (ABIN7874065) specifically detects RS1 in ELISA and WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for RS1 antibody (AA 34-224) (ABIN7874065)

Target: RS1 (Retinoschisin 1 (RS1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RS1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-RS1 Antibody

Binding Specificity: AA 34-224

Purpose: Retinoschisin Antibody / RS1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RS1 recombinant protein (Position: Y34-A224) was used as the immunogen for the Retinoschisin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Retinoschisin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Retinoschisin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RS1

Target: RS1 (Retinoschisin 1 (RS1))

Alternative Name: Retinoschisin

Background: The Retinoschisin antibody targets Retinoschisin, a secreted cell-adhesion protein encoded by the RS1 gene and essential for maintaining the structural integrity of the retina. Retinoschisin is produced primarily by photoreceptor and bipolar cells and forms a disulfide-linked octamer that mediates cell-cell adhesion and retinal layer organization. The Retinoschisin antibody provides a crucial tool for studying retinal development, synaptic architecture, and inherited retinal disorders such as X-linked juvenile retinoschisis (XLRS).

Retinoschisin localizes to the inner surface of photoreceptor and bipolar cell membranes, where it contributes to the formation of organized laminar structures necessary for signal transmission. The Retinoschisin antibody supports visualization of its localization within the outer and inner plexiform layers, providing insights into cell adhesion and extracellular matrix interactions. Through binding to membrane lipids and extracellular partners such as Na/K ATPase and L-type calcium channels, Retinoschisin stabilizes synaptic connectivity and retinal tissue architecture.

Mutations in the RS1 gene result in XLRS, a heritable vitreoretinal dystrophy characterized by splitting of the retinal layers and loss of central vision. The Retinoschisin antibody facilitates research into this condition by detecting the presence or absence of Retinoschisin in patient samples and model systems. Defective or truncated protein forms lead to impaired secretion and defective cell adhesion, causing structural disorganization of the retina and progressive visual impairment.

Beyond its structural role, Retinoschisin influences retinal signaling pathways that regulate photoreceptor synapse function. The Retinoschisin antibody allows for quantitative studies assessing protein levels under different light-adaptation states or disease conditions. In addition, Retinoschisin is being explored as a candidate for gene-replacement therapy, and antibody-based detection is vital for evaluating therapeutic expression and localization.

The Retinoschisin antibody performs effectively in western blotting, immunohistochemistry, and immunofluorescence, yielding punctate staining in retinal layers consistent with extracellular distribution. NSJ Bioreagents provides this antibody as a validated, high-specificity reagent for vision research and molecular ophthalmology. By enabling precise detection of Retinoschisin, the Retinoschisin antibody supports exploration of retinal cell adhesion, synaptic stability, and the molecular pathology of retinal dystrophies.

UniProt: O15537