GLT25D1 antibody (AA 34-454)

Catalog No. ABIN7874081

This Rabbit Polyclonal antibody specifically detects GLT25D1 in WB, ELISA and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GLT25D1 antibody (AA 34-454) (ABIN7874081)

Target: GLT25D1 (Glycosyltransferase 25 Domain Containing 1 (GLT25D1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLT25D1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GLT25D1 Antibody

Binding Specificity: AA 34-454

Purpose: COLGALT1 Antibody / Collagen beta(1-O)galactosyltransferase 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human COLGALT1 recombinant protein (Position: A34-D454) was used as the immunogen for the COLGALT1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the COLGALT1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the COLGALT1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GLT25D1

Target: GLT25D1 (Glycosyltransferase 25 Domain Containing 1 (GLT25D1))

Alternative Name: COLGALT1

Background: COLGALT1 antibody detects Collagen beta(1-O)galactosyltransferase 1, an enzyme that catalyzes the transfer of galactose to hydroxylysine residues in collagen. This post-translational modification is essential for proper collagen folding, stability, and secretion. COLGALT1 functions within the endoplasmic reticulum and Golgi apparatus and contributes to the biosynthesis of structurally mature collagen fibers. The COLGALT1 antibody is widely used in connective tissue, developmental, and fibrosis research to study collagen maturation, glycosylation, and extracellular matrix regulation.

COLGALT1 is encoded by the COLGALT1 gene located on human chromosome 19p13.2. The protein is approximately 739 amino acids long and is a member of the galactosyltransferase family. It forms a functional complex with its homolog COLGALT2 and works sequentially with lysyl hydroxylase (PLOD family) enzymes to glycosylate hydroxylysine residues in collagen alpha chains. This process stabilizes triple helix formation and enhances collagen secretion and cross-linking.

The COLGALT1 antibody detects an 85 kilodalton protein by western blot and shows perinuclear staining under immunofluorescence microscopy consistent with Golgi localization. COLGALT1 activity is required for the synthesis of type I, II, III, and IV collagens. Deficiency or mutation results in defective collagen glycosylation, leading to abnormal extracellular matrix assembly and diseases such as Ehlers-Danlos syndrome and osteogenesis imperfecta-like disorders.

COLGALT1 expression is tightly regulated during development and tissue repair. Increased expression promotes collagen deposition in fibrotic tissues, whereas reduced expression leads to unstable collagen and tissue fragility. It also contributes to tumor stroma remodeling by enhancing extracellular matrix density and rigidity, influencing cancer cell migration and angiogenesis.

As a key enzyme linking collagen biosynthesis and tissue integrity, COLGALT1 serves as a critical target for studying connective tissue disorders and fibrotic diseases. NSJ Bioreagents provides a validated COLGALT1 antibody optimized for its applications, supporting research into collagen glycosylation, fibrosis, and tissue regeneration.

UniProt: Q8NBJ5

Pathways: SARS-CoV-2 Protein Interactome