MAG antibody (AA 34-605)

Catalog No. ABIN7874086

The Mouse Monoclonal anti-MAG antibody (Clone 2G11) (ABIN7874086) specifically detects MAG in WB, FACS, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MAG antibody (AA 34-605) (ABIN7874086)

Target: MAG (Myelin Associated Glycoprotein (MAG))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAG antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 2G11

Product Details anti-MAG Antibody

Binding Specificity: AA 34-605

Purpose: Siglec-4a Antibody / MAG / Myelin-associated glycoprotein

Purification: Affinity purified

Immunogen: A human recombinant partial protein (amino acids E34-R605) was used as the immunogen for the Siglec-4a antibody.

Isotype: IgG2a

Application Details

Application Notes: Optimal dilution of the Siglec-4a antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Siglec-4a antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MAG

Target: MAG (Myelin Associated Glycoprotein (MAG))

Alternative Name: Siglec-4a

Background: MAG (Myelin-associated glycoprotein), also known as Siglec-4a, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney(HEK) cells expressing the NOGO receptor, p75(NTR) was required for MAG-induced intracellular calcium elevation.

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway