Hephaestin antibody (AA 348-1157)

Catalog No. ABIN7874122

The Rabbit Polyclonal anti-Hephaestin antibody (ABIN7874122) specifically detects Hephaestin in WB, ELISA, IHC (p) and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Hephaestin antibody (AA 348-1157) (ABIN7874122)

Target: Hephaestin (HEPH)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hephaestin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-Hephaestin Antibody

Binding Specificity: AA 348-1157

Purpose: Hephaestin Antibody / HEPH

Purification: Antigen affinity purified

Immunogen: A human recombinant partial protein (amino acids E348-Q1157) was used as the immunogen for the Hephaestin antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Hephaestin antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Hephaestin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Hephaestin

Target: Hephaestin (HEPH)

Alternative Name: Hephaestin

Background: Hephaestin (HEPH) is a multicopper ferroxidase that plays a critical role in systemic iron homeostasis. It is primarily expressed in intestinal enterocytes, where it facilitates the export of dietary iron into the bloodstream. Hephaestin functions by oxidizing ferrous iron (Fe2+) to ferric iron (Fe3+), a form that can be bound by transferrin for transport throughout the body. Researchers often use a Hephaestin antibody to study iron metabolism, absorption, and related disorders.

The activity of Hephaestin is closely linked to ferroportin, the only known iron exporter in mammals. By converting Fe2+ to Fe3+, Hephaestin stabilizes iron export and prevents the accumulation of reactive iron species that could cause oxidative stress. Employing a Hephaestin antibody allows scientists to investigate how this protein cooperates with ferroportin and other regulators of iron homeostasis, such as hepcidin and ceruloplasmin.

Mutations in the HEPH gene are associated with iron transport defects and conditions such as anemia and iron overload. Animal models with disrupted Hephaestin function exhibit impaired dietary iron absorption, underscoring its physiological importance. Beyond its intestinal role, Hephaestin has also been detected in other tissues, including the brain and retina, where it may influence neuronal iron balance and oxidative stress responses. Using a Hephaestin antibody supports research into both systemic and tissue-specific aspects of iron regulation.

NSJ Bioreagents provides a high-quality Hephaestin antibody validated for applications such as western blot, immunohistochemistry, and immunofluorescence. Choosing a Hephaestin antibody from NSJ Bioreagents ensures reliable performance and reproducibility in studies of iron transport, metabolism, and disease.

UniProt: Q9BQS7

Pathways: Transition Metal Ion Homeostasis