TRIM23 antibody (AA 35-406)

Catalog No. ABIN7874166

The Rabbit Polyclonal anti-TRIM23 antibody is suitable to detect TRIM23 in samples from Human, Rat and Mouse. It has been validated for WB and ELISA.

Quick Overview for TRIM23 antibody (AA 35-406) (ABIN7874166)

Target: TRIM23 (Tripartite Motif Containing 23 (TRIM23))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM23 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-TRIM23 Antibody

Binding Specificity: AA 35-406

Purpose: TRIM23 Antibody / Tripartite motif-containing protein 23

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human TRIM23 recombinant protein (Position: E35-R406) was used as the immunogen for the TRIM23 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the TRIM23 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the TRIM23 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for TRIM23

Target: TRIM23 (Tripartite Motif Containing 23 (TRIM23))

Alternative Name: TRIM23

Background: TRIM23 antibody detects Tripartite motif-containing protein 23, a multifunctional E3 ubiquitin ligase involved in autophagy, vesicle trafficking, and innate immunity. The UniProt recommended name is Tripartite motif-containing protein 23 (TRIM23). This cytoplasmic protein belongs to the TRIM family, characterized by RING, B-box, and coiled-coil domains mediating protein-protein interactions and ubiquitin ligase activity.

Functionally, TRIM23 antibody identifies a 586-amino-acid protein that regulates signaling complexes and autophagy. TRIM23 ubiquitinates and activates TBK1 kinase, promoting antiviral autophagy and interferon responses. It also coordinates endomembrane trafficking by acting as an Arf GAP (GTPase-activating protein), linking vesicular dynamics to immune signaling.

The TRIM23 gene is located on chromosome 5q12.3 and is expressed in multiple tissues, with highest levels in brain, lung, and immune cells. Through its dual ubiquitin ligase and Arf GAP activities, TRIM23 integrates membrane trafficking and host defense. It contributes to selective autophagy of pathogens and protein aggregates.

Pathologically, altered TRIM23 expression has been associated with neurodegeneration, viral infection, and cancer. In neurons, TRIM23 maintains axonal homeostasis, while in immune cells it regulates autophagic responses. Research using TRIM23 antibody supports studies in innate immunity, autophagy, and vesicle biology.

TRIM23 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect E3 ligases and autophagy regulators. NSJ Bioreagents provides TRIM23 antibody reagents optimized for studies in protein ubiquitination, intracellular trafficking, and immune signaling.

Structurally, Tripartite motif-containing protein 23 features a RING finger domain for E3 ligase activity, B-box and coiled-coil domains for dimerization, and a C-terminal ARF GAP domain. This antibody supports detailed analysis of TRIM23's regulatory roles in autophagy and innate immune signaling.

UniProt: P36406