Occludin antibody (AA 358-520)

Catalog No. ABIN7874285

This Rabbit Polyclonal antibody specifically detects Occludin in WB, IHC, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Occludin antibody (AA 358-520) (ABIN7874285)

Target: Occludin (OCLN)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Occludin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-Occludin Antibody

Binding Specificity: AA 358-520

Purpose: OCLN Antibody / Occludin

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Occludin/OCLN recombinant protein (Position: S358-Q520) was used as the immunogen for the OCLN antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the OCLN antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the OCLN antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Occludin

Target: Occludin (OCLN)

Alternative Name: OCLN

Background: The OCLN antibody targets Occludin, a key integral membrane protein encoded by the OCLN gene that contributes to tight junction formation and epithelial barrier integrity. Occludin is a 59 kDa tetraspan protein localized at intercellular junctions of epithelial and endothelial cells. It works together with claudins, junctional adhesion molecules (JAMs), and cytoplasmic scaffolding proteins such as ZO-1 to form the tight junction complex that regulates paracellular permeability. The OCLN antibody enables specific detection of Occludin, allowing researchers to investigate the molecular architecture of tight junctions and how their disruption contributes to disease.

Occludin plays a central role in maintaining cell polarity, barrier selectivity, and communication between adjacent cells. It acts as both a structural and signaling molecule, participating in pathways that control cell growth and differentiation. Phosphorylation of Occludin on serine, threonine, or tyrosine residues dynamically regulates its interaction with cytoplasmic partners and influences tight junction assembly and disassembly. The OCLN antibody is an essential reagent for tracking these modifications and monitoring Occludin redistribution during epithelial remodeling or inflammation.

Mutations in OCLN cause band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), a neurological disorder associated with cortical malformations and hepatic dysfunction. These findings underscore the systemic importance of Occludin beyond epithelial physiology. The OCLN antibody provides researchers with a means to quantify protein levels in both brain and peripheral tissues, aiding studies into developmental and metabolic roles of tight junction components.

Occludin dysfunction is also implicated in a range of diseases, including cancer metastasis, viral infection, and inflammatory bowel disease. Pathogens such as hepatitis C virus and cytomegalovirus exploit Occludin to gain cellular entry or modulate junction integrity. Altered Occludin expression can weaken epithelial barriers, promoting invasion or chronic inflammation. The OCLN antibody allows visualization of these alterations in tissue models, helping elucidate how tight junction disruption contributes to pathogenesis.

The OCLN antibody performs effectively in immunofluorescence microscopy, immunohistochemistry, and western blotting. In epithelial cultures, staining reveals tight junction localization, while in tissues it highlights barrier organization across endothelium and epithelia. NSJ Bioreagents provides this reagent with validated specificity and reliability for studying Occludin regulation, trafficking, and function. By supporting detailed investigation of tight junction biology, the OCLN antibody helps clarify molecular mechanisms that preserve epithelial homeostasis and their perturbation in human disease.

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C