PNLIP antibody (pan)

Catalog No. ABIN7874313

The Rabbit Polyclonal anti-PNLIP antibody is suitable to detect PNLIP in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF, ICC and FACS.

Quick Overview for PNLIP antibody (pan) (ABIN7874313)

Target: PNLIP (Pancreatic Lipase (PNLIP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNLIP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-PNLIP Antibody

Binding Specificity: AA 36-404, pan

Purpose: PNLIP Antibody / Pancreatic Lipase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Pancreatic Lipase/PNLIP recombinant protein (Position: I36-D404) was used as the immunogen for the PNLIP antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PNLIP antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PNLIP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PNLIP

Target: PNLIP (Pancreatic Lipase (PNLIP))

Alternative Name: PNLIP

Background: PNLIP antibody detects Pancreatic lipase, encoded by the PNLIP gene. Pancreatic lipase is a secreted digestive enzyme essential for hydrolyzing dietary triglycerides into free fatty acids and monoglycerides in the small intestine. PNLIP antibody provides researchers with a critical reagent for studying lipid digestion, gastrointestinal biology, and disorders of fat absorption.

Pancreatic lipase is secreted by the exocrine pancreas into the duodenum, where it functions in concert with bile salts and colipase to emulsify and degrade triglycerides. Research using PNLIP antibody has shown that the enzyme specifically catalyzes the hydrolysis of ester bonds at the sn-1 and sn-3 positions of triglycerides, leaving monoglycerides for absorption. Its central role in fat metabolism underscores its importance for nutritional physiology.

Studies with PNLIP antibody have revealed that deficiency in pancreatic lipase causes steatorrhea and fat malabsorption syndromes, leading to poor nutrient uptake, weight loss, and deficiency of fat-soluble vitamins. Mutations in the PNLIP gene have been linked to congenital pancreatic lipase deficiency, a rare autosomal recessive disorder. In addition, reduced activity is observed in chronic pancreatitis and cystic fibrosis, making pancreatic lipase an important biomarker of pancreatic function.

Beyond digestion, research using PNLIP antibody has highlighted its potential role in metabolic disease. Altered pancreatic lipase activity has been associated with obesity, insulin resistance, and dyslipidemia. Pharmacological inhibitors of pancreatic lipase, such as orlistat, are clinically used to reduce fat absorption in obesity management, emphasizing the therapeutic relevance of this enzyme.

PNLIP antibody is widely used in immunohistochemistry, western blotting, and ELISA. Immunohistochemistry highlights expression in pancreatic acinar cells, western blotting quantifies enzyme levels in pancreatic extracts, and ELISA measures circulating lipase in serum. These applications make PNLIP antibody indispensable for digestive and metabolic research.

By supplying validated PNLIP antibody reagents, NSJ Bioreagents supports studies into lipid metabolism, pancreatic disease, and digestion. Detection of Pancreatic lipase provides researchers with insight into how lipolytic enzymes regulate nutrient absorption and metabolic health.

UniProt: P16233