Neu3 antibody (AA 36-414)

Catalog No. ABIN7874316

The Rabbit Polyclonal anti-Neu3 antibody is suitable to detect Neu3 in samples from Human. It has been validated for ELISA, WB and FACS.

Quick Overview for Neu3 antibody (AA 36-414) (ABIN7874316)

Target: Neu3 (Sialidase 3 (Membrane Sialidase) (Neu3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Neu3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-Neu3 Antibody

Binding Specificity: AA 36-414

Purpose: NEU3 Antibody / Sialidase 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NEU3 recombinant protein (Position: H36-D414) was used as the immunogen for the NEU3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NEU3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NEU3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Neu3

Target: Neu3 (Sialidase 3 (Membrane Sialidase) (Neu3))

Alternative Name: NEU3

Background: NEU3 antibody detects Sialidase-3, a plasma membrane-associated enzyme that catalyzes the hydrolysis of sialic acid residues from gangliosides and glycoproteins. The UniProt recommended name is Sialidase-3 (NEU3). This enzyme is primarily localized at the plasma membrane and regulates signaling processes associated with cell growth, differentiation, and apoptosis through the modulation of membrane sialoglycoconjugates.

Functionally, NEU3 antibody identifies a 428-amino-acid enzyme that preferentially acts on gangliosides such as GM3, GD1a, and GT1b, catalyzing their desialylation. NEU3 activity alters the composition of lipid rafts, influencing membrane receptor function and downstream signaling. It modulates the activity of growth factor receptors, including EGFR and integrins, by affecting their sialic acid content and clustering at the plasma membrane. Through these mechanisms, NEU3 participates in the regulation of cell proliferation, adhesion, and migration.

The NEU3 gene is located on chromosome 11q13.5 and encodes a glycosidase that is anchored to the plasma membrane via hydrophobic domains. NEU3's enzymatic activity contributes to ganglioside catabolism and recycling, which are critical for maintaining lipid membrane homeostasis. Dysregulation of NEU3 expression has been linked to oncogenesis, as elevated levels promote tumor cell survival, motility, and resistance to apoptosis. Conversely, NEU3 deficiency impairs receptor-mediated signaling and disrupts ganglioside balance in neuronal and epithelial cells.

In cancer biology, NEU3 overexpression enhances epidermal growth factor receptor (EGFR) signaling by desialylating GM3, a natural EGFR inhibitor. This results in sustained receptor activation and downstream MAPK and PI3K/AKT pathway stimulation. NEU3 also influences integrin-mediated adhesion and migration, contributing to metastasis. Beyond oncology, NEU3 is involved in immune modulation, neuronal signaling, and muscle differentiation. It also regulates autophagy by modulating membrane glycosphingolipid composition.

NEU3 antibody is widely used in glycobiology, cancer research, and neurobiology to analyze sialidase function and membrane signaling. It supports applications including immunoblotting, immunocytochemistry, and enzyme localization studies. NEU3 expression profiling provides insight into tumor progression, inflammatory signaling, and metabolic disorders. The enzyme's surface localization makes it a potential therapeutic target for modulating receptor activity and glycosphingolipid metabolism.

Structurally, NEU3 contains conserved catalytic motifs typical of sialidases and a hydrophobic transmembrane anchor that facilitates membrane association. It functions as a monomer and is regulated by pH and substrate availability. NSJ Bioreagents provides NEU3 antibody reagents validated for use in membrane biology, oncology, and glycosylation research.

UniProt: Q9UQ49