POLM antibody (AA 36-481)

Catalog No. ABIN7874321

This Rabbit Polyclonal antibody specifically detects POLM in WB, ELISA and IP. It exhibits reactivity toward Human.

Quick Overview for POLM antibody (AA 36-481) (ABIN7874321)

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLM antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-POLM Antibody

Binding Specificity: AA 36-481

Purpose: POLM Antibody / DNA polymerase mu

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human POLM recombinant protein (Position: E36-H481) was used as the immunogen for the POLM antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the POLM antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the POLM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for POLM

Target: POLM (Polymerase (DNA Directed), mu (POLM))

Alternative Name: POLM

Background: POLM antibody detects DNA polymerase mu, a specialized DNA polymerase involved in non-homologous end joining (NHEJ) and DNA repair. The UniProt recommended name is DNA polymerase mu (POLM). This enzyme belongs to the X family of DNA polymerases, functioning at sites of double-strand breaks to fill in short gaps and process damaged DNA ends during repair.

Functionally, POLM antibody identifies a 494-amino-acid nuclear enzyme that exhibits template-dependent and template-independent polymerase activities. POLM incorporates nucleotides opposite damaged or missing bases, ensuring ligation competency of DNA ends. Unlike high-fidelity polymerases, POLM lacks strong proofreading activity, allowing it to tolerate mismatched or gapped templates-a property critical for repair flexibility in NHEJ.

The POLM gene is located on chromosome 7p13 and is expressed in immune and proliferative tissues such as thymus and bone marrow. It plays a key role in V(D)J recombination and class switch recombination in lymphocytes by repairing programmed double-strand breaks. Its activity complements DNA polymerase lambda (POLL) in filling in short DNA overhangs during repair.

Pathologically, dysregulation of POLM contributes to genomic instability and may influence cancer development, immune function, and cellular aging. Overexpression can increase mutation rates, whereas deficiency impairs NHEJ efficiency. POLM's balance between flexibility and error rate is vital for maintaining genome integrity while allowing adaptability in immune diversification.

POLM antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect DNA polymerase mu in nuclear extracts. NSJ Bioreagents provides POLM antibody reagents optimized for DNA repair, recombination, and genomic maintenance research.

Structurally, DNA polymerase mu contains an N-terminal BRCT domain mediating protein-protein interactions, followed by a catalytic polymerase domain with palm, fingers, and thumb subdomains typical of X-family enzymes. This antibody supports studies on POLM's mechanistic role in end joining, mutation repair, and adaptive immunity.

UniProt: Q9NP87

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response