SHE antibody (AA 36-495)

Catalog No. ABIN7874322

The Rabbit Polyclonal anti-SHE antibody is suitable to detect SHE in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC, IF, FACS and ICC.

Quick Overview for SHE antibody (AA 36-495) (ABIN7874322)

Target: SHE (Src Homology 2 Domain Containing E (SHE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SHE antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-SHE Antibody

Binding Specificity: AA 36-495

Purpose: SHE Antibody / SH2 domain-containing adapter protein E

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human SHE recombinant protein (Position: L36-H495) was used as the immunogen for the SHE antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the SHE antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the SHE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SHE

Target: SHE (Src Homology 2 Domain Containing E (SHE))

Alternative Name: SHE

Background: SHE antibody detects SH2 domain-containing adapter protein E, a cytoplasmic signaling adaptor that mediates growth factor and cytokine receptor signaling. Encoded by the SHE gene on chromosome 2p13.3, this protein functions downstream of receptor tyrosine kinases and cytokine receptors, facilitating the recruitment of effector molecules involved in cell growth, differentiation, and survival. By coupling phosphorylated receptors to intracellular signaling cascades, SHE helps coordinate pathways such as MAPK/ERK and PI3K/AKT.

SHE contains a central SH2 domain that binds phosphotyrosine motifs on activated receptors and associated kinases. Through its modular architecture, it acts as a scaffold that brings kinases and transcriptional regulators into close proximity, ensuring precise and timely propagation of receptor-initiated signals. SHE interacts with proteins such as EGFR, JAK2, and STAT3, influencing processes from hematopoietic differentiation to epithelial cell proliferation.

The SHE antibody is widely used in cell signaling, oncology, and hematology research to study adaptor-mediated signal transduction and receptor dynamics. Western blot analysis identifies a 62 kilodalton band corresponding to SHE, while immunofluorescence shows cytoplasmic and perinuclear localization in stimulated cells. This antibody is essential for dissecting receptor coupling events and understanding how adaptor proteins coordinate cellular responses to growth factors.

In cancer and immune regulation, altered SHE expression or phosphorylation disrupts normal signal transduction, leading to abnormal cell proliferation and survival. Overactivation of SHE-associated signaling contributes to tumor progression, while its suppression can impair cytokine responsiveness and differentiation. The SHE antibody provides a sensitive tool for evaluating these effects and monitoring adaptor function in normal and disease contexts. NSJ Bioreagents validates this antibody for its applications, ensuring dependable and reproducible performance for studies on receptor and kinase signaling networks.

UniProt: Q5VZ18