EBI3 antibody (AA 366-466)

Catalog No. ABIN7874364

The Mouse Monoclonal anti-EBI3 antibody is suitable to detect EBI3 in samples from Human. It has been validated for WB, ELISA and IHC (p).

Quick Overview for EBI3 antibody (AA 366-466) (ABIN7874364)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Grade: Carrier-free

Clone: EBI3-8906

Product Details anti-EBI3 Antibody

Binding Specificity: AA 366-466

Purpose: Epstein-Barr virus induced 3 Antibody / EBI3 (azide and preservative free)

Purification: Protein A/G affinity

Immunogen: A recombinant partial protein sequence (within amino acids 366-466) from the human protein was used as the immunogen for the Epstein-Barr virus induced 3 antibody.

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal dilution of the Epstein-Barr virus induced 3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: -20 °C

Storage Comment: Aliquot the Epstein-Barr virus induced 3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: Epstein-Barr virus induced 3

Background: Epstein-Barr virus-induced gene 3 (Ebi3) is a widely expressed homolog to the interleukin IL-12 p40 subunit protein that forms a heterodimer with either IL-12 p35 or an IL-12 p35 homolog, p28, to create a new cytokine (IL-27). IL-27 is an early product of activated antigen-presenting cells and drives rapid clonal expansion of naive but not memory CD4+ T cells. Interferon-b differentially regulates expression of the IL-12 family members p35, p40, p19 and Ebi3 in activated human dendritic cells. Ebi3 may function to antagonize IL-12 and to inhibit the development of a Th1 immune response. Ebi3 is strongly expressed in Hodgkin and Reed-Sternberg cells, independently of the EBV status of the tumor cells. Research suggests that Ebi3 may be an additional component of the repertoire employed by Hodgkin and Reed-Sternberg cells to inhibit an effective anti-tumor or anti-viral immune response. The human Ebi3 gene maps to chromosome 19p13.3 and encodes a secreted glycoprotein that is expressed in spleen and tonsils, and at high levels in full-term placenta.

UniProt: Q14213