PI4KB antibody (AA 375-671)

Catalog No. ABIN7874462

This Rabbit Polyclonal antibody specifically detects PI4KB in WB, ELISA, IHC (p) and FACS. It exhibits reactivity toward Human.

Quick Overview for PI4KB antibody (AA 375-671) (ABIN7874462)

Target: PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PI4KB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-PI4KB Antibody

Binding Specificity: AA 375-671

Purpose: PI4KB Antibody / Phosphatidylinositol 4-kinase beta

Purification: Antigen affinity chromatography

Immunogen: An E.coli-derived human recombinant protein (amino acids D375-D671) was used as the immunogen for the PI4KB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PI4KB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PI4KB Antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PI4KB

Target: PI4KB (Phosphatidylinositol 4-Kinase, Catalytic, beta (PI4KB))

Alternative Name: PI4KB

Background: Phosphatidylinositol 4-kinase beta is an enzyme that in humans is encoded by the PI4KB gene. Inositol phospholipids have an important role in intracellular signaling in response to hormones, growth factors and neurotransmitters. Phosphatidylinositol 4-kinase phosphorylates phosphatidylinositol (PI) to phosphatidylinositol-4-phosphate (PIP). In a second step, PIP is further phosphorylated to phosphatidylinositol-4,5-bisphosphate (PIP2), and PIP2 is subsequently hydrolyzed by phospholipase C, producing the two intracellular second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI4K230, PI4K92 and PI4K55 are three PI4 kinase isoforms that have been characterized and classified according to their molecular weights of 230, 92 and 55 kD. Previously, PI4 kinases were classified into type II and III enzymes. All isoforms are located on distinct membranes and cellular compartments suggesting various tasks. PI4K230 is located at the endoplasmatic reticulum and outer membranes of mitochondria, PI4K92 at the Golgi apparatus and endoplasmatic reticulum, and PI4K55 at the plasma membrane and endosomes. PI4K230 is predominantly expressed in brain and moderately sensitive to wortmannin as well as specifically and irreversibly inhibited by cyclitol derivatives.

UniProt: Q9UBF8

Pathways: Inositol Metabolic Process