MYBBP1A antibody (AA 39-1316)

Catalog No. ABIN7874570

This Rabbit Polyclonal antibody specifically detects MYBBP1A in WB, ELISA, IF and ICC. It exhibits reactivity toward Mouse and Rat.

Quick Overview for MYBBP1A antibody (AA 39-1316) (ABIN7874570)

Target: MYBBP1A (Myb-Binding Protein 1A (MYBBP1A))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MYBBP1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-MYBBP1A Antibody

Binding Specificity: AA 39-1316

Purpose: MYBBP1A Antibody / Myb-binding protein 1A

Purification: Immunogen affinity purified

Immunogen: E.coli-derived mouse MYBBP1A recombinant protein (Position: D39-R1316) was used as the immunogen for the MYBBP1A antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MYBBP1A antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MYBBP1A antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MYBBP1A

Target: MYBBP1A (Myb-Binding Protein 1A (MYBBP1A))

Alternative Name: MYBBP1A

Background: MYBBP1A antibody detects Myb-binding protein 1A, a nucleolar transcriptional regulator that modulates gene expression, chromatin structure, and cell cycle progression. Encoded by the MYBBP1A gene on chromosome 17p13.3, this large nuclear protein was originally identified through its interaction with the Myb oncoprotein, but it also binds multiple transcription factors including p53, NF?B, and STAT3. MYBBP1A contains several acidic and coiled-coil domains that facilitate protein-protein interactions and nucleolar localization sequences that target it to the nucleolus under normal growth conditions. It functions as both a transcriptional coactivator and corepressor, integrating stress responses with cell cycle control.

MYBBP1A plays a key role in ribosome biogenesis and nucleolar stress signaling. Upon cellular stress or energy depletion, MYBBP1A translocates from the nucleolus to the nucleoplasm, where it enhances p53 acetylation and activation, promoting cell cycle arrest and apoptosis. It also suppresses c-Myb-driven transcription to prevent uncontrolled proliferation. Through these mechanisms, MYBBP1A acts as a tumor suppressor and regulator of growth arrest. Depletion of MYBBP1A leads to impaired rRNA processing and increased genomic instability. Dysregulation of its expression has been reported in renal carcinoma, hepatocellular carcinoma, and hematologic malignancies.

The MYBBP1A antibody is used in cancer biology and transcriptional regulation studies to detect Myb-binding protein 1A localization and abundance. Western blot analysis typically identifies a ~150 kilodalton band, while immunofluorescence shows predominant nucleolar staining with redistribution upon stress. Because MYBBP1A interacts with both histone-modifying enzymes and transcriptional machinery, it is a critical factor for understanding nucleolar function and epigenetic regulation. The antibody is effective in chromatin immunoprecipitation, immunohistochemistry, and nuclear fractionation assays. The MYBBP1A antibody therefore supports research on tumor suppression, transcriptional control, and nucleolar biology. NSJ Bioreagents provides this reagent validated for high specificity and reproducibility across applications.

UniProt: Q7TPV4