RAB27B antibody (AA 4-218)

Catalog No. ABIN7874662

The Rabbit Polyclonal anti-RAB27B antibody (ABIN7874662) specifically detects RAB27B in WB, ELISA, IHC, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RAB27B antibody (AA 4-218) (ABIN7874662)

Target: RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAB27B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-RAB27B Antibody

Binding Specificity: AA 4-218

Purpose: RAB27B Antibody / Ras related protein Rab-27B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human RAB27B recombinant protein (Position: G4-C218) was used as the immunogen for the RAB27B antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the RAB27B antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the RAB27B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for RAB27B

Target: RAB27B (RAB27B, Member RAS Oncogene Family (RAB27B))

Alternative Name: RAB27B

Background: RAB27B antibody detects Ras related protein Rab-27B, encoded by the RAB27B gene. Ras related protein Rab-27B is a member of the Rab family of small GTP binding proteins that regulate vesicle trafficking, secretion, and membrane transport. RAB27B antibody provides researchers with a highly specific reagent for studying secretory pathways, exocytosis, and disease processes involving vesicle transport.

Ras related protein Rab-27B localizes to secretory granules and vesicles, where it regulates docking and fusion events. Research using RAB27B antibody has shown that it interacts with effectors such as Munc13-4 and Slac2 proteins, coordinating tethering and priming of vesicles prior to exocytosis. This function is critical for regulated secretion in endocrine, neuronal, and immune cells.

Studies with RAB27B antibody have demonstrated that it plays a role in exocytosis of lysosome-related organelles, platelet dense granules, and lytic granules of cytotoxic T cells. By coordinating vesicle release, Rab-27B contributes to hormone secretion, platelet function, and immune responses. These findings highlight its broad physiological roles in vesicle biology.

Dysregulation of Ras related protein Rab-27B has been associated with cancer and neurological disease. Research using RAB27B antibody has shown that overexpression promotes secretion of growth factors and extracellular vesicles in cancers such as breast and gastric cancer, supporting invasion and metastasis. Variants in RAB27B also influence susceptibility to neurological disorders, where altered secretion affects neuronal communication.

RAB27B antibody is widely applied in immunofluorescence, western blotting, and immunohistochemistry. Immunofluorescence demonstrates vesicular localization, western blotting quantifies expression levels, and immunohistochemistry reveals tissue-specific distribution. These applications make RAB27B antibody indispensable for vesicle trafficking research.

By supplying validated RAB27B antibody reagents, NSJ Bioreagents supports studies into exocytosis, vesicle trafficking, and disease. Detection of Ras related protein Rab-27B provides researchers with insight into how Rab GTPases regulate secretion and intercellular communication.

UniProt: O00194