JMJD5 antibody (AA 4-406)

Catalog No. ABIN7874677

The Rabbit Polyclonal anti-JMJD5 antibody (ABIN7874677) specifically detects JMJD5 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for JMJD5 antibody (AA 4-406) (ABIN7874677)

Target: JMJD5 (Jumonji Domain Containing 5 (JMJD5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMJD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-JMJD5 Antibody

Binding Specificity: AA 4-406

Purpose: KDM8 Antibody / Lysine-specific demethylase 8

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human KDM8 recombinant protein (Position: D4-D406) was used as the immunogen for the KDM8 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the KDM8 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the KDM8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for JMJD5

Target: JMJD5 (Jumonji Domain Containing 5 (JMJD5))

Alternative Name: KDM8

Background: KDM8 antibody detects Lysine-specific demethylase 8, a histone demethylase that regulates chromatin organization, transcription, and cell cycle progression. Encoded by the KDM8 gene on chromosome 16p12.1, this enzyme demethylates dimethylated lysine 36 on histone H3 (H3K36me2), a modification associated with transcriptional elongation and gene regulation. KDM8 plays an essential role in epigenetic control of gene expression, particularly during mitotic progression and differentiation.

KDM8, also known as JMJD5, is a member of the Jumonji C (JmjC) domain-containing family of Fe(II)- and 2-oxoglutarate-dependent oxygenases. It localizes to the nucleus and functions both as a histone demethylase and as a cofactor for cell cycle regulatory proteins such as CDK1. Through these activities, KDM8 coordinates chromatin dynamics and ensures proper metaphase alignment during mitosis.

The KDM8 antibody is widely used in epigenetics, developmental biology, and cancer research to study histone methylation control, transcriptional regulation, and chromatin remodeling. Western blot analysis detects a 47 kilodalton band corresponding to KDM8, while immunofluorescence reveals nuclear staining consistent with its chromatin-associated functions. This antibody enables examination of histone modification patterns and their effects on gene expression and cell division.

Aberrant expression of KDM8 has been linked to oncogenesis, particularly in breast and prostate cancers, where it promotes proliferation and cell cycle progression by modulating chromatin accessibility. KDM8 also interacts with p53 and ribosomal proteins to coordinate metabolic and stress responses. The KDM8 antibody provides a powerful tool for studying epigenetic regulation and transcriptional dynamics. NSJ Bioreagents offers this antibody validated for its applications, ensuring accurate detection in chromatin and gene regulation research.

UniProt: Q8N371

Pathways: Chromatin Binding