NSF antibody (AA 4-636)

Catalog No. ABIN7874683

The Rabbit Polyclonal anti-NSF antibody (ABIN7874683) specifically detects NSF in WB, ELISA, IF, IHC (p) and FACS. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for NSF antibody (AA 4-636) (ABIN7874683)

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Product Details anti-NSF Antibody

Binding Specificity: AA 4-636

Purpose: Vesicle-fusing ATPase Antibody / NSF

Purification: Antigen affinity purified

Immunogen: Recombinant human protein (amino acids R4-Q636) was used as the immunogen for the Vesicle-fusing ATPase antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the Vesicle-fusing ATPase antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the Vesicle-fusing ATPase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NSF

Target: NSF (N-Ethylmaleimide-Sensitive Factor (NSF))

Alternative Name: Vesicle-fusing ATPase

Background: N-ethylmaleimide-sensitive factor, also known as NSF and Vesicle-fusing ATPase, is an enzyme which in humans is encoded by the NSF gene. NSF is a homohexameric AAA ATPase involved in membrane fusion. NSF is ubiquitously found in the cytoplasm of eukaryotic cells. It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another. During this process, SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex. This aids fusion of the vesicle with the target membrane. It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred, using the hydrolysis of ATP as an energy source, allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion. This proposal remains controversial, however. Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane.

UniProt: P46459