NSUN6 antibody (AA 40-400)

Catalog No. ABIN7874698

The Rabbit Polyclonal anti-NSUN6 antibody (ABIN7874698) specifically detects NSUN6 in WB, ELISA, IHC and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for NSUN6 antibody (AA 40-400) (ABIN7874698)

Target: NSUN6 (NOP2/Sun Domain Family, Member 6 (NSUN6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NSUN6 Antibody

Binding Specificity: AA 40-400

Purpose: NSUN6 Antibody / NOP2/Sun RNA methyltransferase family member 6

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NSUN6 recombinant protein (Position: E40-E400) was used as the immunogen for the NSUN6 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NSUN6 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NSUN6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for NSUN6

Target: NSUN6 (NOP2/Sun Domain Family, Member 6 (NSUN6))

Alternative Name: NSUN6

Background: NSUN6 antibody detects NOP2/Sun RNA methyltransferase family member 6, encoded by the NSUN6 gene on chromosome 10q22.1. NSUN6 antibody is widely used in RNA biology, epigenetics, and cancer research. NSUN6 belongs to the RNA cytosine methyltransferase family, catalyzing site-specific methylation of cytosine residues in tRNAs and possibly other RNAs. This modification, known as 5-methylcytosine, influences RNA stability, structure, and function.

Structurally, NSUN6 is a ~55 kDa cytoplasmic protein containing an RNA recognition motif and a conserved catalytic domain typical of cytosine-5 methyltransferases. It selectively modifies tRNAs at position C72 in the acceptor stem, a modification critical for efficient aminoacylation and translation. NSUN6 localizes predominantly in the cytoplasm but may also act in the nucleus under certain conditions.

Functionally, NSUN6 promotes translation fidelity and efficiency by stabilizing tRNA structure. It contributes to cellular stress responses, growth, and differentiation. Altered NSUN6 activity disrupts RNA modifications, impacting protein synthesis and cellular homeostasis. Researchers use NSUN6 antibody to study RNA epigenetics, translation control, and stress biology.

Clinically, dysregulation of RNA methyltransferases including NSUN6 is implicated in cancer, neurodevelopmental disorders, and viral infections. NSUN6 overexpression has been linked to certain tumors, suggesting a role in tumor growth. Conversely, loss-of-function may impair translation and stress adaptation. Because RNA modifications are emerging therapeutic targets, NSUN6 is an attractive focus for research. NSJ Bioreagents supplies NSUN6 antibody for epigenetics, cancer, and RNA biology studies.

Experimentally, NSUN6 antibody is applied in western blotting to detect the ~55 kDa protein, in immunofluorescence microscopy to visualize cytoplasmic distribution, and in RNA immunoprecipitation to map RNA substrates. Co-immunoprecipitation with NSUN6 antibody identifies protein partners in RNA methylation complexes.

UniProt: Q8TEA1