SPERT antibody (AA 40-448)

Catalog No. ABIN7874700

The Rabbit Polyclonal anti-SPERT antibody is suitable to detect SPERT in samples from Human, Rat and Mouse. It has been validated for ELISA, WB and FACS.

Quick Overview for SPERT antibody (AA 40-448) (ABIN7874700)

Target: SPERT (Spermatid Associated (SPERT))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPERT antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-SPERT Antibody

Binding Specificity: AA 40-448

Purpose: CBY2 Antibody / Chibby 2

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human CBY2 recombinant protein (Position: E40-V448) was used as the immunogen for the CBY2 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CBY2 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CBY2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for SPERT

Target: SPERT (Spermatid Associated (SPERT))

Alternative Name: CBY2

Background: CBY2 antibody detects Chibby homolog 2, a centrosome- and cilium-associated protein involved in ciliogenesis, cell polarity, and Wnt signaling regulation. Encoded by the CBY2 gene on chromosome 12q24.13, this protein belongs to the Chibby family, which includes CBY1 and CBY3, known for modulating beta-catenin signaling and ciliary structure. CBY2 contains coiled-coil domains that mediate dimerization and interaction with other ciliary proteins such as CEP164 and ODF2. It localizes to the basal body and transition zone of cilia, where it contributes to the assembly and stabilization of the ciliary axoneme.

CBY2 is expressed in ciliated tissues including respiratory epithelium, reproductive organs, and brain ependyma. It plays an essential role in the early stages of ciliogenesis by promoting docking of basal bodies to the plasma membrane and initiating axoneme extension. Disruption of CBY2 function leads to defective ciliogenesis, altered epithelial polarity, and impaired signaling through cilia-dependent pathways such as Hedgehog and Wnt. In reproductive cells, CBY2 contributes to flagellar formation and sperm motility, while in neurons it supports ependymal cilia function crucial for cerebrospinal fluid flow.

The CBY2 antibody is widely used in cell biology and developmental studies to analyze ciliary formation, centrosomal organization, and beta-catenin signaling. Western blot analysis detects a 42 kilodalton band corresponding to full-length CBY2, and immunofluorescence reveals punctate staining at basal bodies marked by gamma-tubulin. In cilia-deficient models, CBY2 expression serves as a marker for disrupted ciliogenesis. The antibody is also useful for exploring CBY2's role in planar cell polarity and coordination of multiciliated cell differentiation.

Mutations or reduced expression of CBY2 have been linked to ciliopathies, infertility, and hydrocephalus, reflecting its vital role in ciliary assembly. The CBY2 antibody provides a powerful tool for investigating these disorders and for mapping ciliary protein networks in epithelial and germline systems. NSJ Bioreagents supplies this antibody validated for its applications, ensuring reliable results in research on centrosome and cilia biology.

UniProt: Q8NA61