Calpain 3 antibody (AA 42-764)

Catalog No. ABIN7874928

The Rabbit Polyclonal anti-Calpain 3 antibody is suitable to detect Calpain 3 in samples from Human and Mouse. It has been validated for WB and ELISA.

Quick Overview for Calpain 3 antibody (AA 42-764) (ABIN7874928)

Target: Calpain 3 (CAPN3)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Calpain 3 Antibody

Binding Specificity: AA 42-764

Purpose: CAPN3 Antibody / Calpain 3

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human Calpain 3/CAPN3 recombinant protein (Position: I42-D764) was used as the immunogen for the CAPN3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CAPN3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the CAPN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Calpain 3

Target: Calpain 3 (CAPN3)

Alternative Name: CAPN3

Background: CAPN3 antibody detects Calpain 3, a skeletal muscle-specific calcium-dependent cysteine protease involved in muscle remodeling and sarcomere maintenance. The UniProt recommended name is Calpain 3 (CAPN3). This enzyme belongs to the calpain family of proteases that regulate cytoskeletal dynamics and muscle protein turnover.

Functionally, CAPN3 antibody identifies a 821-amino-acid protease characterized by catalytic cysteine and histidine residues forming the proteolytic triad. Calpain 3 associates with titin in the sarcomere and modulates proteolytic remodeling during muscle contraction and regeneration. Its activity is tightly regulated by calcium binding and autolytic activation mechanisms unique to muscle tissue.

The CAPN3 gene is located on chromosome 15q15.1 and is expressed predominantly in skeletal muscle. Calpain 3 acts as a muscle-specific regulator controlling sarcomeric integrity and signaling pathways that respond to mechanical stress and injury. It is rapidly activated upon calcium influx, enabling dynamic structural adaptation.

Pathologically, mutations in CAPN3 cause limb-girdle muscular dystrophy type 2A (LGMD2A), an autosomal recessive disorder characterized by progressive muscle weakness and atrophy. Deficiency of Calpain 3 disrupts sarcomere homeostasis and impairs muscle repair. Research using CAPN3 antibody supports studies in muscle physiology, proteolysis, and dystrophic disease mechanisms.

CAPN3 antibody is validated for western blotting, immunohistochemistry, and immunofluorescence to detect muscle-specific proteases. NSJ Bioreagents provides CAPN3 antibody reagents optimized for studies in muscle biology, calcium signaling, and protein turnover.

Structurally, Calpain 3 consists of protease core domains (PC1 and PC2) flanked by unique insertion sequences IS1 and IS2, which confer muscle-specific regulation. This antibody aids investigation of CAPN3's enzymatic role in sarcomere remodeling and muscular dystrophy.

UniProt: P20807

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development