DIS3 antibody (AA 43-878)

Catalog No. ABIN7874977

The Rabbit Polyclonal anti-DIS3 antibody (ABIN7874977) specifically detects DIS3 in WB, ELISA, IHC, IF, FACS and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for DIS3 antibody (AA 43-878) (ABIN7874977)

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIS3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-DIS3 Antibody

Binding Specificity: AA 43-878

Purpose: DIS3 Antibody / Exosome complex exonuclease RRP44

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human DIS3 recombinant protein (Position: H43-K878) was used as the immunogen for the DIS3 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the DIS3 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the DIS3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for DIS3

Target: DIS3 (Exosome Complex Exonuclease RRP44 (DIS3))

Alternative Name: DIS3

Background: DIS3 antibody detects Exosome complex exonuclease RRP44, a catalytic subunit of the RNA exosome complex responsible for RNA degradation and processing. The UniProt recommended name is Exosome complex exonuclease RRP44 (DIS3). This enzyme functions as both a 3'-5' exoribonuclease and endoribonuclease, ensuring precise control of RNA quality, turnover, and surveillance in eukaryotic cells.

Functionally, DIS3 antibody identifies a 958-amino-acid cytoplasmic and nuclear protein that associates with the multi-protein RNA exosome complex. DIS3 degrades defective, misprocessed, or surplus RNA species, including rRNA, snoRNA, snRNA, and mRNA. It possesses two catalytic domainsi 1/2an RNB domain for exonucleolytic degradation and a PIN domain for endonucleolytic cleavagei 1/2allowing versatile RNA processing under various physiological conditions. By maintaining RNA homeostasis, DIS3 contributes to normal gene expression and cellular viability.

The DIS3 gene is located on chromosome 13q22.1 and is expressed ubiquitously in proliferative tissues. Its activity is regulated through complex assembly, post-translational modification, and subcellular localization. DIS3 operates in both the nucleus, where it processes pre-rRNA and small noncoding RNAs, and the cytoplasm, where it degrades aberrant mRNA species.

Pathologically, mutations in DIS3 cause multiple myeloma, acute myeloid leukemia, and related hematologic malignancies. These mutations impair RNA degradation, leading to accumulation of defective transcripts and deregulated gene expression. Loss of DIS3 function disrupts RNA quality control and contributes to oncogenic transformation. Research using DIS3 antibody supports studies in RNA metabolism, gene regulation, and cancer genomics.

DIS3 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect exosome complex components. NSJ Bioreagents provides DIS3 antibody reagents optimized for research in RNA processing, degradation pathways, and hematologic cancer biology.

Structurally, Exosome complex exonuclease RRP44 contains an N-terminal PIN domain for endonucleolytic activity and a central RNB catalytic core typical of RNase II family enzymes. The C-terminal S1 and CSD domains enable RNA substrate recognition and binding. This antibody aids in investigating DIS3's role in RNA exosome-mediated decay and RNA quality control in normal and malignant cells.

UniProt: Q9Y2L1