CD235b/GYPB antibody (AA 43-91)

Catalog No. ABIN7874978

This Rabbit Polyclonal antibody specifically detects CD235b/GYPB in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for CD235b/GYPB antibody (AA 43-91) (ABIN7874978)

Target: CD235b/GYPB (GYPB) (Glycophorin B (GYPB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD235b/GYPB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD235b/GYPB Antibody

Binding Specificity: AA 43-91

Purpose: GYPB Antibody / Glycophorin B

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GYPB recombinant protein (Position: Q43-A91) was used as the immunogen for the GYPB antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GYPB antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GYPB antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for CD235b/GYPB

Target: CD235b/GYPB (GYPB) (Glycophorin B (GYPB))

Alternative Name: GYPB

Background: GYPB antibody detects Glycophorin B, encoded by the GYPB gene. Glycophorin B is a major sialoglycoprotein of the erythrocyte membrane that contributes to blood group antigen expression, membrane structure, and host-pathogen interactions. GYPB antibody provides researchers with a specific reagent to study red blood cell biology, blood group systems, and disease processes involving erythrocyte surface proteins.

Glycophorin B is a single-pass transmembrane protein heavily glycosylated on its extracellular domain. Research using GYPB antibody has shown that it carries antigens of the MNS blood group system, including the S and s antigens. Variation in GYPB alleles results in polymorphism that defines clinically significant blood group phenotypes. These polymorphisms are essential for transfusion medicine and compatibility testing.

Studies with GYPB antibody have revealed that Glycophorin B contributes to erythrocyte membrane stability. By interacting with the cytoskeleton and forming complexes with other membrane proteins, it maintains cell shape and flexibility. Glycophorin B also provides a negatively charged surface that reduces red blood cell aggregation and contributes to circulation efficiency.

In infectious disease research, Glycophorin B is notable as a receptor for Plasmodium falciparum, the parasite responsible for malaria. Research using GYPB antibody has shown that parasite invasion efficiency depends on interactions with glycophorins, including Glycophorin B. Genetic variants of GYPB influence malaria susceptibility, making this protein highly relevant for host-pathogen interaction studies.

Dysregulation or mutation of GYPB can result in rare blood group phenotypes, which may cause challenges in transfusion compatibility. Studies with GYPB antibody have also suggested potential roles in erythroid development and signaling, although these functions remain less well defined compared to its structural and antigenic roles.

GYPB antibody is widely applied in flow cytometry, immunohistochemistry, and immunoblotting. Flow cytometry distinguishes blood group antigen expression on red cells, immunohistochemistry identifies surface expression patterns, and immunoblotting quantifies protein levels. These applications make GYPB antibody indispensable in transfusion medicine and infectious disease research.

By supplying validated GYPB antibody reagents, NSJ Bioreagents supports studies into erythrocyte biology, blood group systems, and host-pathogen interactions. Detection of Glycophorin B provides researchers with insight into how red cell surface proteins contribute to health and disease.

UniProt: P06028