GLYAT antibody (AA 44-296)

Catalog No. ABIN7875013

The Rabbit Polyclonal anti-GLYAT antibody (ABIN7875013) specifically detects GLYAT in WB, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for GLYAT antibody (AA 44-296) (ABIN7875013)

Target: GLYAT (Glycine N-Acyltransferase (GLYAT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLYAT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-GLYAT Antibody

Binding Specificity: AA 44-296

Purpose: GLYAT Antibody / Glycine-N-acyltransferase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human GLYAT recombinant protein (Position: K44-L296) was used as the immunogen for the GLYAT antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the GLYAT antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the GLYAT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for GLYAT

Target: GLYAT (Glycine N-Acyltransferase (GLYAT))

Alternative Name: GLYAT

Background: GLYAT antibody detects Glycine-N-acyltransferase, encoded by the GLYAT gene on chromosome 11q12.1. GLYAT antibody is widely used to study this phase II conjugating enzyme involved in detoxification and metabolism of acyl-CoA thioesters. GLYAT catalyzes conjugation of glycine to acyl-CoA substrates, producing acylglycines that are more water-soluble and easily excreted. Expression is highest in liver and kidney, the major detoxification organs, but it is also present in brain and other tissues involved in metabolic processing.

Structurally, GLYAT is a mitochondrial matrix enzyme belonging to the acyltransferase family. It contains conserved motifs for CoA binding and catalytic activity. Structural studies suggest GLYAT functions as a homodimer, with active sites capable of accommodating medium- to long-chain acyl-CoA substrates. This structural adaptability allows it to process a wide range of xenobiotic and endogenous compounds.

Functionally, GLYAT contributes to detoxification of acyl-CoA intermediates generated during fatty acid, amino acid, and xenobiotic metabolism. By conjugating glycine to these intermediates, GLYAT prevents their accumulation and toxicity. Acylglycines produced by GLYAT can serve as diagnostic markers of inborn errors of metabolism, including organic acidemias. Knockdown studies demonstrate impaired detoxification capacity and increased cellular stress. Researchers use GLYAT antibody to study metabolic regulation, detoxification, and biomarker pathways.

Clinically, GLYAT deficiency or reduced activity is associated with metabolic disorders characterized by elevated toxic acyl-CoA levels. Acylglycine profiling is used in newborn screening for metabolic diseases such as isovaleric acidemia. GLYAT expression has also been linked to cancer, where metabolic reprogramming alters detoxification pathways. Additionally, GLYAT is implicated in drug metabolism and may influence individual responses to xenobiotics. NSJ Bioreagents offers GLYAT antibody as a research tool in toxicology, metabolism, and disease studies.

Experimentally, GLYAT antibody is applied in western blotting to detect the ~34 kDa protein, in immunofluorescence microscopy to confirm mitochondrial localization, and in immunohistochemistry to analyze tissue expression. Enzymatic assays combined with GLYAT antibody detection help characterize acyltransferase activity under different conditions.

UniProt: Q6IB77