MSTO1 antibody (AA 44-518)

Catalog No. ABIN7875020

This Rabbit Polyclonal antibody specifically detects MSTO1 in WB, ELISA, IHC, FACS and IF. It exhibits reactivity toward Human.

Quick Overview for MSTO1 antibody (AA 44-518) (ABIN7875020)

Target: MSTO1 (Misato Homolog 1 (MSTO1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MSTO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-MSTO1 Antibody

Binding Specificity: AA 44-518

Purpose: MSTO1 Antibody / Misato homolog 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human MSTO1 recombinant protein (Position: E44-Q518) was used as the immunogen for the MSTO1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the MSTO1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the MSTO1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for MSTO1

Target: MSTO1 (Misato Homolog 1 (MSTO1))

Alternative Name: MSTO1

Background: MSTO1 antibody detects Misato homolog 1, a cytoplasmic protein involved in mitochondrial morphology, fusion, and intracellular distribution. The UniProt recommended name is Misato homolog 1 (MSTO1). This evolutionarily conserved protein plays a critical role in maintaining mitochondrial network integrity and energy homeostasis, particularly in tissues with high metabolic demand.

Functionally, MSTO1 antibody identifies a 570-amino-acid cytoplasmic protein that promotes mitochondrial fusion by regulating the dynamics of the mitochondrial outer and inner membranes. MSTO1 interacts with components of the mitochondrial fusion machinery, including mitofusins (MFN1 and MFN2) and OPA1, coordinating mitochondrial elongation and connectivity. By promoting mitochondrial network formation, MSTO1 supports oxidative phosphorylation, calcium buffering, and apoptotic resistance.

The MSTO1 gene is located on chromosome 1q22 and encodes a protein expressed in muscle, liver, brain, and fibroblasts. MSTO1 is partially localized to the cytosol and associates dynamically with mitochondrial membranes. Its loss-of-function mutations cause mitochondrial fragmentation, reduced ATP production, and increased reactive oxygen species, leading to neurodegeneration and myopathy. MSTO1 deficiency has been linked to a rare inherited condition characterized by developmental delay, muscle weakness, and optic atrophy.

In cellular metabolism, MSTO1 contributes to the maintenance of mitochondrial homeostasis during cell division and stress. It facilitates mitochondrial fusion after fission events, ensuring the proper distribution of mitochondrial DNA and metabolic components. Overexpression of MSTO1 enhances mitochondrial interconnectivity, while depletion disrupts network morphology and compromises cell viability. These effects highlight MSTO1 as a key regulator of mitochondrial quality control.

MSTO1 antibody is widely used in mitochondrial biology, metabolic research, and neuromuscular disease studies. It is suitable for western blotting, immunocytochemistry, and fluorescence microscopy to detect MSTO1 localization and expression. This antibody supports studies of mitochondrial dynamics, fusion-fission balance, and bioenergetic regulation. In translational research, MSTO1 serves as a molecular marker of mitochondrial health and metabolic adaptation.

Structurally, MSTO1 contains coiled-coil and helical domains that facilitate protein-protein interaction and oligomerization. It functions as a cytoplasmic tether promoting mitochondrial membrane juxtaposition. NSJ Bioreagents provides MSTO1 antibody reagents validated for use in mitochondrial dynamics, fusion regulation, and energy metabolism research.

UniProt: Q9BUK6