Polybromo 1 antibody (AA 45-1647)

Catalog No. ABIN7875057

The Rabbit Polyclonal anti-Polybromo 1 antibody is suitable to detect Polybromo 1 in samples from Human, Mouse and Rat. It has been validated for WB, FACS and ELISA.

Quick Overview for Polybromo 1 antibody (AA 45-1647) (ABIN7875057)

Target: Polybromo 1 (PBRM1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Polybromo 1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-Polybromo 1 Antibody

Binding Specificity: AA 45-1647

Purpose: PBRM1 Antibody / Polybromo 1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PBRM1 recombinant protein (Position: D45-R1647) was used as the immunogen for the PBRM1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PBRM1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PBRM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for Polybromo 1

Target: Polybromo 1 (PBRM1)

Alternative Name: PBRM1

Background: PBRM1 antibody detects Polybromo-1, a chromatin-remodeling factor and tumor suppressor that regulates gene expression through nucleosome repositioning. The UniProt recommended name is Polybromo-1 (PBRM1). This large nuclear protein functions as a subunit of the PBAF (polybromo-associated BRG1-associated factor) subtype of the SWI/SNF chromatin-remodeling complex, which modulates chromatin accessibility to control transcription, differentiation, and DNA repair.

Functionally, PBRM1 antibody identifies a 1,709-amino-acid protein containing six bromodomains, two BAH domains, and one HMG-box domain that recognize acetylated lysine residues on histones. These interactions recruit the PBAF complex to specific promoters, enabling ATP-dependent chromatin remodeling and transcriptional regulation. PBRM1 supports cellular homeostasis by maintaining chromatin architecture and transcriptional fidelity.

The PBRM1 gene is located on chromosome 3p21.1 and is highly expressed in kidney, liver, and lung tissues. It acts as a critical tumor suppressor by opposing oncogenic transcriptional programs, particularly in renal and hepatic cells.

Pathologically, PBRM1 mutations are frequently observed in clear cell renal cell carcinoma (ccRCC), intrahepatic cholangiocarcinoma, and other malignancies. Loss of PBRM1 disrupts chromatin remodeling, leading to transcriptional dysregulation and uncontrolled cell proliferation. Research using PBRM1 antibody supports studies in epigenetics, tumor suppression, and chromatin dynamics.

PBRM1 antibody is validated for western blotting, immunoprecipitation, and immunohistochemistry to detect chromatin-remodeling complex components. NSJ Bioreagents provides PBRM1 antibody reagents optimized for transcriptional regulation, chromatin accessibility, and cancer epigenomics research.

Structurally, Polybromo-1 features multiple bromodomains that bind acetylated histones and a modular organization that promotes assembly with PBAF complex members such as BRD7 and SMARCA4. This antibody facilitates exploration of PBRM1's role in chromatin organization and gene silencing.

UniProt: Q86U86