FOXM1 antibody (AA 457-760)

Catalog No. ABIN7875105

The Rabbit Polyclonal anti-FOXM1 antibody is suitable to detect FOXM1 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IP.

Quick Overview for FOXM1 antibody (AA 457-760) (ABIN7875105)

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXM1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-FOXM1 Antibody

Binding Specificity: AA 457-760

Purpose: FOXM1 Antibody / Forkhead box protein M1

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human FOXM1 recombinant protein (Position: Q457-P760) was used as the immunogen for the FOXM1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the FOXM1 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the FOXM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for FOXM1

Target: FOXM1 (Forkhead Box M1 (FOXM1))

Alternative Name: FOXM1

Background: FOXM1 antibody detects Forkhead box protein M1, a transcription factor that regulates cell cycle progression, DNA replication, and mitotic gene expression. Encoded by the FOXM1 gene on chromosome 12p13.33, this nuclear protein belongs to the Forkhead family of transcription factors, characterized by a conserved winged-helix DNA-binding domain. FOXM1 functions as a key regulator of cell proliferation by activating genes required for G1/S and G2/M phase transitions, including cyclins, PLK1, and CDC25B.

FOXM1 localizes to the nucleus during interphase and associates with condensed chromosomes during mitosis. Its activity is controlled by phosphorylation through CDKs and PLK1, which modulate DNA-binding and transcriptional activation. FOXM1 also contributes to chromatin remodeling and genomic stability by facilitating DNA repair and replication stress responses. It serves as an essential factor in tissue regeneration, stem cell renewal, and oncogenic transformation.

The FOXM1 antibody is widely used in cancer, developmental, and cell cycle research to investigate proliferation control and transcriptional regulation. Western blot analysis detects a 90 kilodalton band corresponding to FOXM1, while immunofluorescence shows strong nuclear staining in proliferating cells. This antibody enables assessment of mitotic activity and transcriptional control mechanisms in both normal and cancerous tissues.

Overexpression of FOXM1 is a hallmark of many human cancers, where it promotes tumor growth, angiogenesis, and metastasis through activation of cell cycle and invasion-related genes. Its inhibition is being explored as a therapeutic strategy for targeting rapidly dividing cells. The FOXM1 antibody provides a reliable tool for studying transcriptional regulation of proliferation and tumorigenesis. NSJ Bioreagents validates this antibody for its applications, ensuring accurate and reproducible detection for transcriptional and oncogenic research.

UniProt: Q08050

Pathways: Positive Regulation of Response to DNA Damage Stimulus