APOA1BP antibody (AA 46-288)

Catalog No. ABIN7875118

The Rabbit Polyclonal anti-APOA1BP antibody (ABIN7875118) specifically detects APOA1BP in WB, IHC, ELISA and FACS. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for APOA1BP antibody (AA 46-288) (ABIN7875118)

Target: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APOA1BP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Product Details anti-APOA1BP Antibody

Binding Specificity: AA 46-288

Purpose: NAXE Antibody / NAD(P)HX epimerase

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human NAXE recombinant protein (Position: R46-Q288) was used as the immunogen for the NAXE antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the NAXE antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the NAXE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for APOA1BP

Target: APOA1BP (Apolipoprotein A-I Binding Protein (APOA1BP))

Alternative Name: NAXE

Background: The NAXE antibody targets NAD(P)HX epimerase, an enzyme encoded by the NAXE gene that catalyzes the interconversion of the R and S epimers of NADHX and NADPHX. These damaged forms of NADH and NADPH arise through hydration of nicotinamide cofactors under stress conditions. NAD(P)HX epimerase acts together with NAD(P)HX dehydratase to repair these inactive metabolites, maintaining cofactor homeostasis and protecting cells from metabolic imbalance. The NAXE antibody enables precise detection of this enzyme in studies of cofactor metabolism, mitochondrial function, and cellular stress response.

NAD(P)HX epimerase localizes to both the cytosol and mitochondria, reflecting its role in multiple redox compartments. It is essential for the NAD(P)HX repair system, which safeguards the integrity of cellular redox reactions by regenerating functional NADH and NADPH. The NAXE antibody allows researchers to analyze this protective mechanism, revealing how cells maintain coenzyme pools under conditions of heat, oxidative, or metabolic stress. Loss of NAXE function leads to accumulation of toxic NADHX derivatives, disrupting energy metabolism and enzymatic activity.

Mutations in the NAXE gene cause a rare neurometabolic disorder characterized by recurrent encephalopathy and neurodegeneration, particularly following febrile illness. The NAXE antibody supports mechanistic studies into this disease, helping identify tissue-specific expression and enzyme deficiency in patient-derived samples. Defective NAD(P)HX epimerase activity compromises mitochondrial respiration and redox balance, underscoring its essential role in cellular survival.

Beyond its role in repair metabolism, NAD(P)HX epimerase contributes to stress adaptation and antioxidant defense. It ensures a continuous supply of NAD(P)H required for reductive biosynthesis and detoxification pathways. The NAXE antibody supports quantitative analyses of enzyme regulation in models of oxidative stress, aging, and metabolic disorders. Dysregulation of NAD(P)HX repair enzymes has also been linked to impaired neuronal function and energy deficiency syndromes.

The NAXE antibody performs effectively in western blotting, immunofluorescence, and immunohistochemistry, providing characteristic mitochondrial and cytosolic staining. NSJ Bioreagents provides this antibody with validated specificity and reproducibility for biochemistry, metabolism, and neuroscience research. By enabling accurate detection of NAD(P)HX epimerase, the NAXE antibody advances understanding of cofactor repair, mitochondrial redox regulation, and neuroprotective metabolic pathways.

UniProt: Q8NCW5