HBZ antibody (AA 48-142)

Catalog No. ABIN7875179

This Rabbit Polyclonal antibody specifically detects HBZ in WB and ELISA. It exhibits reactivity toward Rat and Mouse.

Quick Overview for HBZ antibody (AA 48-142) (ABIN7875179)

Target: HBZ (Hemoglobin, zeta (HBZ))

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HBZ antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-HBZ Antibody

Binding Specificity: AA 48-142

Purpose: HBZ Antibody / Hemoglobin zeta

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human HBZ recombinant protein (Position: D48-R142) was used as the immunogen for the HBZ antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the HBZ antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the HBZ antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for HBZ

Target: HBZ (Hemoglobin, zeta (HBZ))

Alternative Name: HBZ

Background: HBZ antibody detects Hemoglobin subunit zeta, encoded by the HBZ gene on chromosome 16p13.3. HBZ antibody is widely used in hematology, developmental biology, and globin gene regulation research. Hemoglobin zeta is an embryonic alpha-like globin expressed during early development in yolk sac-derived erythrocytes. It participates in embryonic hemoglobins (Hb Portland-1 and Hb Portland-2) that provide oxygen transport before fetal hemoglobins are expressed. Expression of HBZ is normally silenced after embryonic development as gamma and beta globins become dominant.

Structurally, Hemoglobin zeta is a ~16 kDa globin protein that forms heterotetramers with epsilon and gamma globins during early development. Its globin fold binds heme, enabling reversible oxygen binding. The protein sequence is highly homologous to alpha globin, reflecting its evolutionary relationship. Despite being developmentally restricted, HBZ can be re-expressed in certain pathologies.

Functionally, Hemoglobin zeta provides oxygen transport in the embryonic circulation and supports survival of primitive erythrocytes. Its transient expression is essential for early development. Researchers use HBZ antibody to investigate globin gene switching, embryonic hematopoiesis, and hemoglobinopathies.

Clinically, persistence of Hemoglobin zeta expression has been observed in some alpha-thalassemias and hematological malignancies. HBZ reactivation may contribute to altered oxygen transport or serve as a biomarker of erythroid stress. Genetic disorders affecting HBZ expression impact early embryonic viability. NSJ Bioreagents provides HBZ antibody for developmental hematology and globin research.

Experimentally, HBZ antibody is used in western blotting to detect the ~16 kDa protein, in immunohistochemistry to study embryonic tissue expression, and in ELISA to quantify hemoglobin zeta in blood samples. Molecular assays with HBZ antibody support studies of globin gene regulation.

UniProt: P02008