PUF60 antibody (AA 48-559)

Catalog No. ABIN7875211

The Rabbit Polyclonal anti-PUF60 antibody (ABIN7875211) specifically detects PUF60 in WB, IHC, ELISA, IP and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for PUF60 antibody (AA 48-559) (ABIN7875211)

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUF60 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-PUF60 Antibody

Binding Specificity: AA 48-559

Purpose: PUF60 Antibody / Poly(U)-binding splicing factor 60 kDa

Purification: Immunogen affinity purified

Immunogen: E.coli-derived human PUF60 recombinant protein (Position: Q48-A559) was used as the immunogen for the PUF60 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the PUF60 antibody should be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: After reconstitution, the PUF60 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Target Details for PUF60

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Alternative Name: PUF60

Background: PUF60 antibody detects Poly(U)-binding splicing factor 60 kDa, an RNA-binding protein that regulates pre-mRNA splicing and transcriptional control. The UniProt recommended name is Poly(U)-binding splicing factor 60 kDa (PUF60). This multifunctional protein binds uridine-rich RNA sequences and facilitates spliceosome assembly by promoting U2 snRNP recruitment to introns.

Functionally, PUF60 antibody identifies a 548-amino-acid protein containing two RNA recognition motifs (RRMs) that mediate specific binding to pre-mRNA and single-stranded DNA. PUF60 enhances exon definition and alternative splicing by interacting with U2AF65, SF3B1, and other splicing factors. It also cooperates with transcriptional regulators to coordinate RNA processing and gene expression.

The PUF60 gene is located on chromosome 8q24.3 and is ubiquitously expressed, with high levels in brain, testis, and proliferative tissues. PUF60 acts as a bridge between transcription and RNA processing machinery, ensuring accurate and efficient mRNA maturation.

Pathologically, mutations or haploinsufficiency of PUF60 cause developmental syndromes characterized by intellectual disability, short stature, and craniofacial abnormalities. Abnormal PUF60 expression has also been associated with cancer and splicing-related disorders. Research using PUF60 antibody supports studies in RNA metabolism, splicing regulation, and genetic disease mechanisms.

PUF60 antibody is validated for western blotting, immunoprecipitation, and immunofluorescence to detect splicing factors and RNA-binding proteins. NSJ Bioreagents provides PUF60 antibody reagents optimized for transcriptional and post-transcriptional regulation research.

Structurally, Poly(U)-binding splicing factor 60 kDa contains tandem RRMs and a glycine-rich C-terminal region that facilitate RNA recognition and spliceosome assembly. This antibody enables exploration of PUF60's role in RNA splicing and gene expression control.

UniProt: Q9UHX1